PDBsum entry 3v98

Oxidoreductase

PDB id: 3v98

Contents

Protein chain

669 a.a.

Metals

FE2 ×2

Waters ×956

PDB id:

3v98

Name:

Oxidoreductase

Title:

S663d stable-5-lox

Structure:

Arachidonate 5-lipoxygenase. Chain: a, b. Synonym: 5-lo, 5-lipoxygenase. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: alox5, log5. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.07Å R-factor: 0.167 R-free: 0.197

Authors:

N.C.Gilbert,Z.Rui,D.B.Neau,M.Waight,S.G.Bartlett,W.E.Boeglin, A.R.Brash,M.E.Newcomer

Key ref:

N.C.Gilbert et al. (2012). Conversion of human 5-lipoxygenase to a 15-lipoxygenase by a point mutation to mimic phosphorylation at Serine-663. Faseb J, 26, 3222-3229. PubMed id: 22516296

Date:

23-Dec-11 Release date: 02-May-12

Protein chains

P09917  (LOX5_HUMAN) -  Polyunsaturated fatty acid 5-lipoxygenase from Homo sapiens

Seq: 674 a.a.

Struc: 669 a.a.*

* PDB and UniProt seqs differ at 12 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.1.13.11.- - ?????
• Enzyme class 3: E.C.1.13.11.34 - arachidonate 5-lipoxygenase.

Pathway:

• Reaction: (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = leukotriene A4 + H2O
• Cofactor: Fe cation

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Faseb J 26:3222-3229 (2012)

PubMed id: 22516296

Conversion of human 5-lipoxygenase to a 15-lipoxygenase by a point mutation to mimic phosphorylation at Serine-663.

N.C.Gilbert, Z.Rui, D.B.Neau, M.T.Waight, S.G.Bartlett, W.E.Boeglin, A.R.Brash, M.E.Newcomer.

ABSTRACT

No abstract given.