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PDBsum entry 3v91
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PDB id:
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Transferase
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Title:
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Structure of t82m glycogenin mutant truncated at residue 270 complexed with udp-glucose
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Structure:
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Glycogenin-1. Chain: a. Fragment: unp residues 1-271. Synonym: gn-1, gn1. Engineered: yes. Mutation: yes
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Source:
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Oryctolagus cuniculus. European rabbit,japanese white rabbit,domestic rabbit,rabbits. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.00Å
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R-factor:
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0.208
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R-free:
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0.236
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Authors:
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M.E.Carrizo,J.M.Romero,F.M.Issoglio,J.A.Curtino
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Key ref:
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M.E.Carrizo
et al.
(2012).
Structural and biochemical insight into glycogenin inactivation by the glycogenosis-causing T82M mutation.
Febs Lett,
586,
254-257.
PubMed id:
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Date:
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23-Dec-11
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Release date:
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25-Jan-12
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PROCHECK
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Headers
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References
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P13280
(GLYG_RABIT) -
Glycogenin-1 from Oryctolagus cuniculus
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Seq:
Struc:
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333 a.a.
256 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.2.4.1.186
- glycogenin glucosyltransferase.
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Reaction:
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1.
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L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L- tyrosyl-[glycogenin] + UDP + H+
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2.
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[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D- glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H+
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L-tyrosyl-[glycogenin]
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+
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UDP-alpha-D-glucose
Bound ligand (Het Group name = )
corresponds exactly
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=
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alpha-D-glucosyl-L- tyrosyl-[glycogenin]
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+
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UDP
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+
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H(+)
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[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin]
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+
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UDP-alpha-D- glucose
Bound ligand (Het Group name = )
corresponds exactly
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=
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[1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin]
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+
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UDP
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+
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H(+)
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Cofactor:
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Mn(2+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Febs Lett
586:254-257
(2012)
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PubMed id:
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Structural and biochemical insight into glycogenin inactivation by the glycogenosis-causing T82M mutation.
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M.E.Carrizo,
J.M.Romero,
F.M.Issoglio,
J.A.Curtino.
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ABSTRACT
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Links
