EC 2.4.1.186 - glycogenin glucosyltransferase.

_EC 2.4.1.186 glycogenin glucosyltransferase.

41 PDB entries

EC 2.-.-.- Transferases. [47,784 PDB entries]

EC 2.4.-.- Glycosyltransferases. [3,858 PDB entries]

EC 2.4.1.- Hexosyltransferases. [1,832 PDB entries]

EC 2.4.1.186 glycogenin glucosyltransferase. [41 PDB entries]

1ll0

Reaction: (1) L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L- tyrosyl-[glycogenin] + Udp + H(+). (2) [1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D- glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + Udp + H(+).

L-tyrosyl-[glycogenin]
+
UDP-alpha-D-glucose
= alpha-D-glucosyl-L- tyrosyl-[glycogenin]
+
UDP
+ H(+)

[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin]
+
UDP-alpha-D- glucose
= [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin]
+
UDP
+ H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): glycogenin. priming glucosyltransferase.

Comments: The first reaction of this enzyme is to catalyze its own glucosylation, normally at a specific Tyr of the protein if this group is free; when the Tyr is replaced by Thr or Phe, the enzyme's self-glucosylation activity is lost but its intermolecular transglucosylation ability remains. It continues to glucosylate an existing glucosyl group until a length of about 5-13 residues has been formed. Further lengthening of the glycogen chain is then carried out by Ec 2.4.1.11. Not highly specific for the donor, using UDP-xylose in addition to

Links: [IntEnz] [ExPASy] [KEGG]

There are 41 PDB entries in enzyme class E.C.2.4.1.186

PDB code Protein

1ll0

Crystal structure of rabbit muscle glycogenin

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Tissue: skeletal muscle. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Chains: A, B, C, D, E, F, G, H, I, J (264 residues) CATH domain: 3.90.550.10

1ll2

Crystal structure of rabbit muscle glycogenin complexed with udp- glucose and manganese

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Tissue: skeletal muscle. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Chain: A (255 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

1ll3

Crystal structure of rabbit muscle glycogenin

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Tissue: skeletal muscle. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Chain: A (261 residues) CATH domain: 3.90.550.10

1zct

Structure of glycogenin truncated at residue 270 in a complex with udp

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Chains: A, B (258 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

1zcu

Apo form of the 162s mutant of glycogenin

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Chain: A (260 residues) CATH domain: 3.90.550.10

1zcv

Apo form of a mutant of glycogenin in which asp159 is replaced by asn

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Chain: A (260 residues) CATH domain: 3.90.550.10

1zcy

Apo form of a mutant of glycogenin in which asp159 is replaced by ser

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Chain: A (257 residues) CATH domain: 3.90.550.10

1zdf

Ser162 mutant of glycogenin complexed with udp-glucose and manganese

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Chain: A (258 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

1zdg

Ser159 mutant of glycogenin complexed with udp-glucose and manganese

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Chain: A (258 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

3q4s

Crystal structure of human glycogenin-1 (gyg1), apo form

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chain: A (251 residues) CATH domain: 3.90.550.10

3qvb

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese and udp

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chain: A (251 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3rmv

Crystal structure of human glycogenin-1 (gyg1) t83m mutant complexed with manganese and udp

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chain: A (249 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3rmw

Crystal structure of human glycogenin-1 (gyg1) t83m mutant complexed with manganese and udp-glucose

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chain: A (250 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

3t7m

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese and udp, in a triclinic closed form

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chains: A, B (263 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3t7n

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese and udp, in a monoclinic closed form

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chains: A, B (254 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3t7o

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese, udp-glucose and glucose

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chains: A, B (263 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

3u2t

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chain: A (256 residues) CATH domain: 3.90.550.10

3u2u

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese, udp and maltotetraose

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B (263 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3u2v

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese, udp and maltohexaose

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Chains: A, B (263 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3u2w

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese and glucose or a glucal species

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Chains: A, B (262 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3u2x

Crystal structure of human glycogenin-1 (gyg1) complexed with manganese, udp and 1'-deoxyglucose

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B (263 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3usq

Structure of d159s/y194f glycogenin mutant truncated at residue 270

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (256 residues) CATH domain: 3.90.550.10

3usr

Structure of y194f glycogenin mutant truncated at residue 270

Source: Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (259 residues) CATH domain: 3.90.550.10

3v8y

Structure of apo-glycogenin truncated at residue 270

Source: Oryctolagus cuniculus. European rabbit,japanese white rabbit,domestic rabbit,rabbits. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (259 residues) CATH domain: 3.90.550.10

3v8z

Structure of apo-glycogenin truncated at residue 270 complexed with udp

Source: Oryctolagus cuniculus. European rabbit,japanese white rabbit,domestic rabbit,rabbits. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (256 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

3v90

Structure of t82m glycogenin mutant truncated at residue 270

Source: Oryctolagus cuniculus. European rabbit,japanese white rabbit,domestic rabbit,rabbits. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (260 residues) CATH domain: 3.90.550.10

3v91

Structure of t82m glycogenin mutant truncated at residue 270 complexed with udp-glucose

Source: Oryctolagus cuniculus. European rabbit,japanese white rabbit,domestic rabbit,rabbits. Organism_taxid: 9986. Gene: gyg, gyg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chain: A (256 residues) CATH domain: 3.90.550.10

Bound ligands: Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

Het Group UPG corresponds to enzyme reactant UDP-alpha-D-glucose

4qlb

Structural basis for the recruitment of glycogen synthase by glycogenin

Source: Caenorhabditis elegans. Roundworm. Organism_taxid: 6239. Gene: gsy-1, y46g5a.31. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: this sequence of gyg-1 (amino acids 268-302) occurs naturally in c. Elegans

Chains: G, E, H, F (34 residues)

4ueg

Crystal structure of human glycogenin-2 catalytic domain

Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: r3-prare2.

Chains: A, B (242 residues) CATH domain: 3.90.550.10

6eqj

Crystal structure of human glycogenin-1 (gyg1) tyr195piphe mutant, apo form

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg1, gyg. Expressed in: escherichia coli. Expression_system_taxid: 562

Chain: A (245 residues)

6eql

Crystal structure of human glycogenin-1 (gyg1) tyr195piphe mutant complexed with manganese and udp

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg1, gyg. Expressed in: escherichia coli. Expression_system_taxid: 562

Chains: A, B (255 residues)

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

7ovx

E3 ring ligase binding domain

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: trim7, gnip, rnf90. Expressed in: escherichia phage ecszw-2. Expression_system_taxid: 2419741. Synthetic: yes. Organism_taxid: 9606

Chain: Q (12 residues)

7q0b

Human gys1-gyg1 complex inhibited state

Source: Homo sapiens. Human. Organism_taxid: 9606. Tissue: muscle. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gyg1, gyg. Expression_system_taxid: 7108

Chains: E, F, G, H (33 residues)

7q0s

Human gys1-gyg1 complex inhibited-like state bound to glucose-6- phosphate

Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: muscel. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Tissue: muscle. Gene: gyg1, gyg.

Chains: E, F, G, H (33 residues)

Bound ligands: Het Group G6P is 46.43% similar to enzyme product UDP

Het Group G6P is 46.43% similar to enzyme product UDP

7q12

Human gys1-gyg1 complex activated state bound to glucose-6-phosphate

Source: Homo sapiens. Human. Organism_taxid: 9606. Tissue: muscle. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gyg1, gyg. Expression_system_taxid: 7108

Chains: E, F, G, H (32 residues)

Bound ligands: Het Group G6P is 46.43% similar to enzyme product UDP

Het Group G6P is 46.43% similar to enzyme product UDP

7q13

Human gys1-gyg1 complex activated state bound to glucose-6-phosphate, uridine diphosphate, and glucose

Source: Homo sapiens. Human. Organism_taxid: 9606. Tissue: muscle. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gyg1, gyg. Expression_system_taxid: 7108

Chains: E, F, G, H (32 residues)

Bound ligands: Het Group UDP corresponds to enzyme product UDP

Het Group UDP corresponds to enzyme product UDP

7zbn

Cryo-em structure of the human gs-gn complex in the inhibited state

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gys1, gys. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: gyg1, gyg. Expression_system_taxid: 7111

Chains: E, F, G, H (33 residues)

8cvx

Human glycogenin-1 and glycogen synthase-1 complex in the presence of glucose-6-phosphate

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: gyg1, gyg.

Chains: E, H, G, F (33 residues)

Bound ligands: Het Group G6P is 46.43% similar to enzyme product UDP

Het Group G6P is 46.43% similar to enzyme product UDP

8cvy

Human glycogenin-1 and glycogen synthase-1 complex in the apo mobile state

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: gyg1, gyg.

Chains: E, G, H (33 residues)

8cvz

Human glycogenin-1 and glycogen synthase-1 complex in the apo ordered state

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gyg1, gyg. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: gys1, gys.

Chains: J, E, I, F, G, H (261 residues)

8z0a

Human gys1-gyg2 complex (apo)

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gys1, gys. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gyg2. Expression_system_taxid: 7108

Chains: E, F, G, H (32 residues)