PDBsum entry 3v3b

Ligase/ligase inhibitor

PDB id: 3v3b

Contents

Protein chains

87 a.a.

13 a.a.

Ligands

THR-PHE-0EH-ASN-LEU-TRP-ARG-LEU-LEU-MK8

Metals

_CL ×8

Waters ×146

PDB id:

3v3b

Name:

Ligase/ligase inhibitor

Title:

Structure of the stapled p53 peptide bound to mdm2

Structure:

E3 ubiquitin-protein ligase mdm2. Chain: a, b. Synonym: double minute 2 protein, hdm2, oncoprotein mdm2, p53-binding protein mdm2. Engineered: yes. Sah-p53-8 stapled-peptide. Chain: c, d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mdm2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic cyclic peptide

Resolution:

2.00Å R-factor: 0.168 R-free: 0.216

Authors:

S.Baek,P.S.Kutchukian,G.L.Verdine,R.Huber,T.A.Holak,L.Ki Won, G.M.Popowicz

Key ref:

S.Baek et al. (2012). Structure of the stapled p53 peptide bound to Mdm2. J Am Chem Soc, 134, 103-106. PubMed id: 22148351

Date:

13-Dec-11 Release date: 18-Jan-12

Protein chains

Q00987  (MDM2_HUMAN) -  E3 ubiquitin-protein ligase Mdm2 from Homo sapiens

Seq: 491 a.a.

Struc: 87 a.a.

Protein chain

No UniProt id for this chain

Struc: 13 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

J Am Chem Soc 134:103-106 (2012)

PubMed id: 22148351

Structure of the stapled p53 peptide bound to Mdm2.

S.Baek, P.S.Kutchukian, G.L.Verdine, R.Huber, T.A.Holak, K.W.Lee, G.M.Popowicz.

ABSTRACT

No abstract given.