PDBsum entry 3s8h

Lyase

PDB id: 3s8h

Contents

Protein chains

292 a.a.

Ligands

3OH ×2

Waters ×219

PDB id:

3s8h

Name:

Lyase

Title:

Structure of dihydrodipicolinate synthase complexed with 3- hydroxypropanoic acid(hpa)at 2.70 a resolution

Structure:

Dihydrodipicolinate synthase. Chain: a, b. Synonym: dhdps. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Strain: pao1. Gene: dapa. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.70Å R-factor: 0.214 R-free: 0.242

Authors:

M.Kumar,N.Kaur,S.Kumar,M.Sinha,P.Kaur,S.Sharma,T.P.Singh

Key ref:

M.Kumar et al. Structure of dihydrodipicolinate synthase complexed w 3-Hydroxypropanoic acid(hpa)at 2.70 a resolution. To be published, .

Date:

28-May-11 Release date: 15-Jun-11

Protein chains

Q9I4W3  (DAPA_PSEAE) -  4-hydroxy-tetrahydrodipicolinate synthase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 292 a.a.

Struc: 292 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.3.3.7 - 4-hydroxy-tetrahydrodipicolinate synthase.
• Reaction: L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5- tetrahydrodipicolinate + H2O + H⁺

L-aspartate 4-semialdehyde + pyruvate (Bound ligand (Het Group name = 3OH) matches with 71.43% similarity) = (2S,4S)-4-hydroxy-2,3,4,5- tetrahydrodipicolinate + H2O + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site