UniProt functional annotation for Q9I4W3

UniProt code: Q9I4W3.

Organism:		Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.
Function:		Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). {ECO:0000305|PubMed:21396954}.
Catalytic activity:		Reaction=L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy- 2,3,4,5-tetrahydrodipicolinate + H(+) + H2O; Xref=Rhea:RHEA:34171, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:67139, ChEBI:CHEBI:537519; EC=4.3.3.7; Evidence={ECO:0000255|HAMAP-Rule:MF_00418};
Biophysicochemical properties:		Kinetic parameters: KM=0.90 mM for pyruvate {ECO:0000269|PubMed:21396954}; KM=0.17 mM for L-aspartate-4-semialdehyde {ECO:0000269|PubMed:21396954}; Vmax=0.030 mmol/min/ug enzyme {ECO:0000269|PubMed:21396954}; pH dependence: Optimum pH is 8.0. At pH 9.0 or above, only 30% activity is observed. {ECO:0000269|PubMed:21396954}; Temperature dependence: Optimum temperature is 37 degrees Celsius. The activity decreases slowly when temperatures are reduced from 37 degrees Celsius while the drop in activity is relatively more rapid when temperatures are raised above 37 degrees Celsius. In this case, the activity reduces to almost 30% at 52 degrees Celsius. {ECO:0000269|PubMed:21396954};
Pathway:		Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. {ECO:0000255|HAMAP-Rule:MF_00418}.
Subunit:		Homodimer. {ECO:0000255|HAMAP-Rule:MF_00418, ECO:0000269|PubMed:21396954}.
Subcellular location:		Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00418}.
Similarity:		Belongs to the DapA family. {ECO:0000255|HAMAP- Rule:MF_00418}.

Annotations taken from UniProtKB at the EBI.