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PDBsum entry 3s4x
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Hydrolase PDB id
3s4x

 

 

 

 

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Contents
Protein chain
367 a.a.
Ligands
SO4 ×7
Waters ×221
PDB id:
3s4x
Name: Hydrolase
Title: Crystal structure of the asn152gly mutant of p99 beta-lactamase
Structure: Beta-lactamase. Chain: a. Synonym: cephalosporinase. Engineered: yes. Mutation: yes
Source: Enterobacter cloacae. Organism_taxid: 550. Strain: p99. Gene: ampc. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.95Å     R-factor:   0.179     R-free:   0.234
Authors: J.F.Ruble,R.A.Powers
Key ref: J.F.Ruble et al. (2012). Structural analysis of the Asn152Gly mutant of P99 cephalosporinase. Acta Crystallogr D Biol Crystallogr, 68, 1189-1193. PubMed id: 22948919
Date:
20-May-11     Release date:   01-Aug-12    
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P05364  (AMPC_ENTCL) -  Beta-lactamase from Enterobacter cloacae
Seq:
Struc: 		381 a.a.
367 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.5.2.6  - beta-lactamase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway: 		Penicillin Biosynthesis and Metabolism
      Reaction: a beta-lactam + H2O = a substituted beta-amino acid
      Cofactor: Zn(2+)

 

 
Acta Crystallogr D Biol Crystallogr 68:1189-1193 (2012)
PubMed id: 22948919  
 
 
Structural analysis of the Asn152Gly mutant of P99 cephalosporinase.
J.F.Ruble, S.T.Lefurgy, V.W.Cornish, R.A.Powers.
 
  ABSTRACT  
 
No abstract given.

 

 

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