PDBsum entry 3pph

Transferase

PDB id: 3pph

Contents

Protein chains

756 a.a.

Waters ×51

PDB id:

3pph

Name:

Transferase

Title:

Crystal structure of the candida albicans methionine synthase by surface entropy reduction, threonine variant

Structure:

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase. Chain: a, b. Synonym: cobalamin-independent methionine synthase, methionine synthase, vitamin-b12 independent isozyme. Engineered: yes. Mutation: yes

Source:

Candida albicans. Yeast. Organism_taxid: 5476. Strain: bwp17. Gene: cao19.10083, cao19.2551, met6. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.80Å R-factor: 0.213 R-free: 0.276

Authors:

D.Ubhi,K.Kavanagh,A.F.Monzingo,J.D.Robertus

Key ref:

D.Ubhi et al. (2011). Structure of Candida albicans methionine synthase determined by employing surface residue mutagenesis. Arch Biochem Biophys, 513, 19-26. PubMed id: 21689631

Date:

24-Nov-10 Release date: 12-Oct-11

Protein chains

P82610  (METE_CANAL) -  5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase from Candida albicans (strain SC5314 / ATCC MYA-2876)

Seq: 767 a.a.

Struc: 756 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.1.1.14 - 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase.
• Reaction: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
• Cofactor: Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Arch Biochem Biophys 513:19-26 (2011)

PubMed id: 21689631

Structure of Candida albicans methionine synthase determined by employing surface residue mutagenesis.

D.Ubhi, K.L.Kavanagh, A.F.Monzingo, J.D.Robertus.

ABSTRACT

No abstract given.