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PDBsum entry 3okc
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Contents |
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* Residue conservation analysis
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PDB id:
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Transferase
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Title:
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Crystal structure of corynebacterium glutamicum pimb' bound to gdp (orthorhombic crystal form)
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Structure:
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Gdp-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase. Chain: a. Synonym: guanosine diphosphomannose-phosphatidyl-inositol alpha- mannosyltransferase, phosphatidylinositol alpha-mannosyltransferase, pi alpha-mannosyltransferase, alpha-mannosyltransferase, alpha-mant, alpha-d-mannose-alpha-(1-6)-phosphatidylmyo-inositol- mannosyltransferase. Engineered: yes
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Source:
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Corynebacterium glutamicum. Brevibacterium flavum. Organism_taxid: 1718. Gene: cg2400, cgl2186, ncgl2106, pimb. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.40Å
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R-factor:
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0.192
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R-free:
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0.236
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Authors:
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S.M.Batt,T.Jabeen,G.S.Besra,K.Futterer
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Key ref:
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S.M.Batt
et al.
(2010).
Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.
J Biol Chem,
285,
37741-37752.
PubMed id:
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Date:
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24-Aug-10
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Release date:
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15-Sep-10
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PROCHECK
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Headers
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References
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Q8NNK8
(PIMB_CORGL) -
GDP-mannose-dependent monoacylated alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
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Seq:
Struc:
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381 a.a.
378 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.2.4.1.346
- phosphatidyl-myo-inositol dimannoside synthase.
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Reaction:
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1.
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a 1,2-diacyl-sn-glycero-3-phospho-[alpha-D-mannopyranosyl-(1<->6)-D- myo-inositol] + GDP-alpha-D-mannose = a 2,6-O-bis(alpha-D- mannopyranosyl)-1-phosphatidyl-1D-myo-inositol + GDP + H+
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2.
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a 1,2-diacyl-sn-glycero-3-phospho-[alpha-D-6-acyl-mannopyranosyl- (1<->6)-D-myo-inositol] + GDP-alpha-D-mannose = a 2-O-(alpha-D-mannosyl)- 6-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol + GDP + H+
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1,2-diacyl-sn-glycero-3-phospho-[alpha-D-mannopyranosyl-(1<->6)-D- myo-inositol]
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+
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GDP-alpha-D-mannose
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=
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2,6-O-bis(alpha-D- mannopyranosyl)-1-phosphatidyl-1D-myo-inositol
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+
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GDP
Bound ligand (Het Group name = )
corresponds exactly
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H(+)
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1,2-diacyl-sn-glycero-3-phospho-[alpha-D-6-acyl-mannopyranosyl- (1<->6)-D-myo-inositol]
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+
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GDP-alpha-D-mannose
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=
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2-O-(alpha-D-mannosyl)- 6-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
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+
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GDP
Bound ligand (Het Group name = )
corresponds exactly
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+
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H(+)
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Cofactor:
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Mg(2+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Biol Chem
285:37741-37752
(2010)
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PubMed id:
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Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.
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S.M.Batt,
T.Jabeen,
A.K.Mishra,
N.Veerapen,
K.Krumbach,
L.Eggeling,
G.S.Besra,
K.Fütterer.
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ABSTRACT
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Links
