UniProt functional annotation for Q8NNK8



	UniProt functional annotation for Q8NNK8

UniProt code: Q8NNK8.

Organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025).

Taxonomy: Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae; Corynebacterium.

Function: Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM) (PubMed:18421567, PubMed:18178556, PubMed:19395496). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (PubMed:18178556, PubMed:19395496). PimB also catalyzes the addition of a mannosyl residue from GDP-Man to the position 6 of phosphatidyl-myo-inositol bearing an acylated alpha-1,2-linked mannose residue (Ac1PIM1) to generate monoacylated phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (By similarity). The addition of the second mannosyl residue by PimB preferentially occurs before the acylation of the mannosyl residue transferred by PimA (By similarity). Also able to transfer a mannosyl residue from GDP-Man to the position 6 of a phosphatidyl-myo-inositol (PI), but this reaction is very slow (By similarity). {ECO:0000250|UniProtKB:A0R043, ECO:0000269|PubMed:18178556, ECO:0000269|PubMed:18421567, ECO:0000269|PubMed:19395496}.

Catalytic activity: Reaction=a 1,2-diacyl-sn-glycero-3-phospho-[alpha-D-mannopyranosyl- (1<->6)-D-myo-inositol] + GDP-alpha-D-mannose = a 2,6-O-bis(alpha-D- mannopyranosyl)-1-phosphatidyl-1D-myo-inositol + GDP + H(+); Xref=Rhea:RHEA:52440, ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189, ChEBI:CHEBI:87673, ChEBI:CHEBI:136624; EC=2.4.1.346; Evidence={ECO:0000305|PubMed:19395496};

Catalytic activity: Reaction=a 1,2-diacyl-sn-glycero-3-phospho-[alpha-D-6-acyl- mannopyranosyl-(1<->6)-D-myo-inositol] + GDP-alpha-D-mannose = 2-O- (alpha-D-mannosyl)-6-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D- myo-inositol + GDP + H(+); Xref=Rhea:RHEA:52444, ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189, ChEBI:CHEBI:88053, ChEBI:CHEBI:136625; EC=2.4.1.346; Evidence={ECO:0000305|PubMed:19395496};

Pathway: Phospholipid metabolism; phosphatidylinositol metabolism. {ECO:0000305|PubMed:19395496}.

Disruption phenotype: Cells lacking this gene are unable to produce monoacylated phosphatidyl-myo-inositol dimannoside (Ac1PIM2) resulting in the accumulation of Ac1PIM1, the absence of LAM and LM-A, and the presence of LM-B. {ECO:0000269|PubMed:18178556, ECO:0000269|PubMed:18421567}.

Similarity: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025).
Taxonomy:		Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae; Corynebacterium.



Function:		Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM) (PubMed:18421567, PubMed:18178556, PubMed:19395496). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (PubMed:18178556, PubMed:19395496). PimB also catalyzes the addition of a mannosyl residue from GDP-Man to the position 6 of phosphatidyl-myo-inositol bearing an acylated alpha-1,2-linked mannose residue (Ac1PIM1) to generate monoacylated phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (By similarity). The addition of the second mannosyl residue by PimB preferentially occurs before the acylation of the mannosyl residue transferred by PimA (By similarity). Also able to transfer a mannosyl residue from GDP-Man to the position 6 of a phosphatidyl-myo-inositol (PI), but this reaction is very slow (By similarity). {ECO:0000250\|UniProtKB:A0R043, ECO:0000269\|PubMed:18178556, ECO:0000269\|PubMed:18421567, ECO:0000269\|PubMed:19395496}.


Catalytic activity:		Reaction=a 1,2-diacyl-sn-glycero-3-phospho-[alpha-D-mannopyranosyl- (1<->6)-D-myo-inositol] + GDP-alpha-D-mannose = a 2,6-O-bis(alpha-D- mannopyranosyl)-1-phosphatidyl-1D-myo-inositol + GDP + H(+); Xref=Rhea:RHEA:52440, ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189, ChEBI:CHEBI:87673, ChEBI:CHEBI:136624; EC=2.4.1.346; Evidence={ECO:0000305\|PubMed:19395496};
Catalytic activity:		Reaction=a 1,2-diacyl-sn-glycero-3-phospho-[alpha-D-6-acyl- mannopyranosyl-(1<->6)-D-myo-inositol] + GDP-alpha-D-mannose = 2-O- (alpha-D-mannosyl)-6-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D- myo-inositol + GDP + H(+); Xref=Rhea:RHEA:52444, ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189, ChEBI:CHEBI:88053, ChEBI:CHEBI:136625; EC=2.4.1.346; Evidence={ECO:0000305\|PubMed:19395496};
Pathway:		Phospholipid metabolism; phosphatidylinositol metabolism. {ECO:0000305\|PubMed:19395496}.
Disruption phenotype:		Cells lacking this gene are unable to produce monoacylated phosphatidyl-myo-inositol dimannoside (Ac1PIM2) resulting in the accumulation of Ac1PIM1, the absence of LAM and LM-A, and the presence of LM-B. {ECO:0000269\|PubMed:18178556, ECO:0000269\|PubMed:18421567}.
Similarity:		Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. {ECO:0000305}.