PDBsum entry 3o1h

Signaling protein

PDB id: 3o1h

Contents

Protein chains

270 a.a.

296 a.a.

Ligands

TMO

Waters ×71

PDB id:

3o1h

Name:

Signaling protein

Title:

Crystal structure of the tors sensor domain - tort complex in the presence of tmao

Structure:

Sensor protein tors. Chain: a. Fragment: sensor domain (unp residues 51-322). Engineered: yes. Periplasmic protein tort. Chain: b. Fragment: unp residues 31-329. Engineered: yes

Source:

Vibrio parahaemolyticus. Organism_taxid: 670. Strain: eb10. Gene: tors, vpa0675. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: tort, vpa0674.

Resolution:

3.10Å R-factor: 0.218 R-free: 0.252

Authors:

J.O.Moore,W.A.Hendrickson

Key ref:

J.O.Moore and W.A.Hendrickson (2012). An asymmetry-to-symmetry switch in signal transmission by the histidine kinase receptor for TMAO. Structure, 20, 729-741. PubMed id: 22483119

Date:

21-Jul-10 Release date: 21-Dec-11

Protein chain

Q87ID1  (Q87ID1_VIBPA) -  histidine kinase from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

Seq: 988 a.a.

Struc: 270 a.a.*

Protein chain

Q87ID2  (Q87ID2_VIBPA) -  Periplasmic protein TorT from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

Seq: 334 a.a.

Struc: 296 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.13.3 - histidine kinase.
• Reaction: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Structure 20:729-741 (2012)

PubMed id: 22483119

An asymmetry-to-symmetry switch in signal transmission by the histidine kinase receptor for TMAO.

J.O.Moore, W.A.Hendrickson.

ABSTRACT

No abstract given.