PDBsum entry 3nq6

Lyase/lyase inhibitor

PDB id: 3nq6

Contents

Protein chains

218 a.a.

Ligands

UP6 ×2

GOL

Waters ×359

PDB id:

3nq6

Name:

Lyase/lyase inhibitor

Title:

Crystal structure of the mutant p180a of orotidine 5'-monophosphate decarboxylase from methanobacterium thermoautotrophicum complexed with inhibitor 6-azaump

Structure:

Orotidine 5'-phosphate decarboxylase. Chain: a, b. Synonym: omp decarboxylase, ompdcase, ompdecase. Engineered: yes. Mutation: yes

Source:

Methanothermobacter thermautotrophicus. Organism_taxid: 145262. Gene: pyrf, mth_129. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.49Å R-factor: 0.171 R-free: 0.194

Authors:

A.A.Fedorov,E.V.Fedorov,B.M.Wood,J.A.Gerlt,S.C.Almo

Key ref:

A.A fedorov et al. Crystal structure of the mutant p180a of orotidine 5'-Monophosphate decarboxylase from methanobacterium thermoautotrophicum complexed with inhibitor 6-Azaump. To be published, .

Date:

29-Jun-10 Release date: 11-May-11

Protein chains

O26232  (PYRF_METTH) -  Orotidine 5'-phosphate decarboxylase from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

Seq: 228 a.a.

Struc: 218 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.1.1.23 - orotidine-5'-phosphate decarboxylase.
• Pathway: Pyrimidine Biosynthesis
• Reaction: orotidine 5'-phosphate + H⁺ = UMP + CO2

orotidine 5'-phosphate + H(+) (Bound ligand (Het Group name = UP6) matches with 80.00% similarity) = UMP + CO2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site