PDBsum entry 3nh4

Hydrolase

PDB id: 3nh4

Contents

Protein chain

303 a.a. *

Ligands

ACT

FMT

Metals

_ZN

_CS ×2

_CL ×2

Waters ×95

* Residue conservation analysis

PDB id:

3nh4

Name:

Hydrolase

Title:

Crystal structure of murine aminoacylase 3

Structure:

Aspartoacylase-2. Chain: a. Synonym: aminoacylase-3, acy-3, aminoacylase iii, acylase iii, hepatitis c virus core-binding protein 1, hcbp1. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: acy-3, acy3, aspa2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.186 R-free: 0.207

Authors:

J.M.Hsieh,K.Tsirulnikov,M.R.Sawaya,N.Magilnick,N.Abuladze,I.Kurtz, J.Abramson,A.Pushkin

Key ref:

J.M.Hsieh et al. (2010). Structures of aminoacylase 3 in complex with acetylated substrates. Proc Natl Acad Sci U S A, 107, 17962-17967. PubMed id: 20921362

Date:

14-Jun-10 Release date: 20-Oct-10

Protein chain

Q91XE4  (ACY3_MOUSE) -  N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming) from Mus musculus

Seq: 318 a.a.

Struc: 303 a.a.

Enzyme reactions

• Enzyme class: E.C.3.5.1.114 - N-acyl-aromatic-L-amino acid amidohydrolase.
• Reaction:
  1. an N-acyl-aromatic L-alpha-amino acid + H2O = an aromatic L-alpha- amino acid + a carboxylate
  2. an N-acetyl-L-cysteine-S-conjugate + H2O = an S-substituted L-cysteine + acetate

N-acyl-aromatic L-alpha-amino acid + H2O = aromatic L-alpha- amino acid (Bound ligand (Het Group name = ACT) matches with 60.00% similarity) + carboxylate

N-acetyl-L-cysteine-S-conjugate + H2O = S-substituted L-cysteine (Bound ligand (Het Group name = ACT) corresponds exactly) + acetate

• Cofactor: Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 107:17962-17967 (2010)

PubMed id: 20921362

Structures of aminoacylase 3 in complex with acetylated substrates.

J.M.Hsieh, K.Tsirulnikov, M.R.Sawaya, N.Magilnick, N.Abuladze, I.Kurtz, J.Abramson, A.Pushkin.

ABSTRACT

No abstract given.