|
|
||
|
|
|
|
|
UniProt functional annotation for Q91XE4 | ||
|
|
|
|
|
|
||
| UniProt code: Q91XE4. |
|
||
| Organism: | |
Mus musculus (Mouse). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. |
|
||
|
||
| Function: | |
Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids. {ECO:0000269|PubMed:14656720, ECO:0000269|PubMed:17012540}. |
|
||
| Catalytic activity: | |
Reaction=an N-acyl-aromatic L-alpha-amino acid + H2O = a carboxylate + an aromatic L-alpha-amino acid; Xref=Rhea:RHEA:54184, ChEBI:CHEBI:15377, ChEBI:CHEBI:29067, ChEBI:CHEBI:84824, ChEBI:CHEBI:138093; EC=3.5.1.114; |
| Catalytic activity: | |
Reaction=an N-acetyl-L-cysteine-S-conjugate + H2O = acetate + an S- substituted L-cysteine; Xref=Rhea:RHEA:36855, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:58717, ChEBI:CHEBI:58718; EC=3.5.1.114; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:20921362}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:20921362}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.1 mM for S-benzyl-N-acetyl-L-cysteine {ECO:0000269|PubMed:14656720}; KM=1.8 mM for N-acetyl-L-histidine {ECO:0000269|PubMed:14656720}; KM=1.4 mM for N-acetyl-L-tyrosine {ECO:0000269|PubMed:14656720}; KM=1.6 mM for N-acetyl-L-phenylalanine {ECO:0000269|PubMed:14656720}; Vmax=11.7 umol/min/mg enzyme with S-benzyl-N-acetyl-L-cysteine as substrate {ECO:0000269|PubMed:14656720}; Vmax=5.9 umol/min/mg enzyme with N-acetyl-L-histidine as substrate {ECO:0000269|PubMed:14656720}; Vmax=7.5 umol/min/mg enzyme with N-acetyl-L-tyrosine as substrate {ECO:0000269|PubMed:14656720}; Vmax=7.9 umol/min/mg enzyme with N-acetyl-L-phenylalanine as substrate {ECO:0000269|PubMed:14656720}; pH dependence: Optimum pH is 7.5 with S-benzyl-N-acetyl-L-cysteine as substrate, 7.6 with N-acetyl-L-histidine as substrate, 7.6 with N-acetyl-L-tyrosine as substrate, and 7.7 with N-acetyl-L-phenylalanine as substrate. {ECO:0000269|PubMed:14656720}; |
| Subunit: | |
Exists as a mixture of homodimers and homotetramer, both catalytically active. {ECO:0000269|PubMed:14656720, ECO:0000269|PubMed:17434493, ECO:0000269|PubMed:20921362}. |
| Subcellular location: | |
Apical cell membrane {ECO:0000269|PubMed:14656720}; Peripheral membrane protein {ECO:0000269|PubMed:14656720}. Cytoplasm {ECO:0000269|PubMed:14656720}. Note=Predominantly localized in the apical membrane of cells in the S1 segment. In the proximal straight tubules (S2 and S3 segments) is expressed diffusely throughout the cytoplasm. |
| Tissue specificity: | |
Expressed predominantly in kidney and to a lesser extent in liver. Weakly expressed in heart, small intestine, brain, lung, testis, and stomach. {ECO:0000269|PubMed:14656720}. |
| Similarity: | |
Belongs to the AspA/AstE family. Aspartoacylase subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.