PDBsum entry 3n4i

Hydrolase/hydrolase inhibitor

PDB id: 3n4i

Contents

Protein chains

265 a.a.

165 a.a.

Waters ×362

PDB id:

3n4i

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of the shv-1 d104e beta-lactamase/beta-lactamase inhibitor protein (blip) complex

Structure:

Beta-lactamase shv-1. Chain: a. Synonym: pit-2. Engineered: yes. Mutation: yes. Beta-lactamase inhibitory protein. Chain: b. Synonym: blip. Engineered: yes

Source:

Klebsiella pneumoniae. Organism_taxid: 573. Gene: bla, bla shv1, rsmk03643, rsmk03800, shv1. Expressed in: escherichia coli. Expression_system_taxid: 562. Streptomyces clavuligerus. Organism_taxid: 1901. Gene: bli. Expression_system_taxid: 469008.

Resolution:

1.56Å R-factor: 0.171 R-free: 0.179

Authors:

M.S.Hanes,K.A.Reynolds,C.Mcnamara,P.Ghosh,R.A.Bonomo,J.F.Kirsch, T.M.Handel

Key ref:

M.S.Hanes et al. (2011). Specificity and cooperativity at β-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactions. Proteins, 79, 1267-1276. PubMed id: 21294157

Date:

21-May-10 Release date: 09-Mar-11

Protein chain

P0AD64  (BLA1_KLEPN) -  Beta-lactamase SHV-1 from Klebsiella pneumoniae

Seq: 286 a.a.

Struc: 265 a.a.*

Protein chain

P35804  (BLIP_STRCL) -  Beta-lactamase inhibitory protein from Streptomyces clavuligerus

Seq: 201 a.a.

Struc: 165 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)

Proteins 79:1267-1276 (2011)

PubMed id: 21294157

Specificity and cooperativity at β-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactions.

M.S.Hanes, K.A.Reynolds, C.McNamara, P.Ghosh, R.A.Bonomo, J.F.Kirsch, T.M.Handel.

ABSTRACT

No abstract given.