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277 a.a.
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100 a.a.
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202 a.a.
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247 a.a.
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* Residue conservation analysis
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PDB id:
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Immune system
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Title:
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Crystal structure of cf34 tcr in complex with hla-b8/flr
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Structure:
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Hla class i histocompatibility antigen, b-8 alpha chain. Chain: a, f. Synonym: mhc class i antigen b 8. Engineered: yes. Beta-2-microglobulin. Chain: b, g. Synonym: beta-2-microglobulin form pi 5.3. Engineered: yes. Flrgraygl peptide from an ebv protein.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: hla-b, hlab. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: b2m, cdabp0092, hdcma22p. Synthetic: yes. Synthetic construct.
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Resolution:
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2.80Å
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R-factor:
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0.223
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R-free:
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0.268
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Authors:
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S.Gras,S.R.Burrows,L.Kjer-Nielsen,C.S.Clements,Y.C.Liu,L.C.Sullivan, A.G.Brooks,A.W.Purcell,J.Mccluskey,J.Rossjohn
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Key ref:
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S.Gras
et al.
(2009).
The shaping of T cell receptor recognition by self-tolerance.
Immunity,
30,
193-203.
PubMed id:
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Date:
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03-Dec-08
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Release date:
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27-Jan-09
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PROCHECK
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Headers
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References
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P01889
(1B07_HUMAN) -
HLA class I histocompatibility antigen, B alpha chain from Homo sapiens
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Seq:
Struc:
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362 a.a.
277 a.a.*
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P61769
(B2MG_HUMAN) -
Beta-2-microglobulin from Homo sapiens
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Seq:
Struc:
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119 a.a.
100 a.a.*
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Immunity
30:193-203
(2009)
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PubMed id:
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The shaping of T cell receptor recognition by self-tolerance.
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S.Gras,
S.R.Burrows,
L.Kjer-Nielsen,
C.S.Clements,
Y.C.Liu,
L.C.Sullivan,
M.J.Bell,
A.G.Brooks,
A.W.Purcell,
J.McCluskey,
J.Rossjohn.
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ABSTRACT
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During selection of the T cell repertoire, the immune system navigates the
subtle distinction between self-restriction and self-tolerance, yet how this is
achieved is unclear. Here we describe how self-tolerance toward a trans-HLA
(human leukocyte antigen) allotype shapes T cell receptor (TCR) recognition of
an Epstein-Barr virus (EBV) determinant (FLRGRAYGL). The recognition of
HLA-B8-FLRGRAYGL by two archetypal TCRs was compared. One was a publicly
selected TCR, LC13, that is alloreactive with HLA-B44; the other, CF34, lacks
HLA-B44 reactivity because it arises when HLA-B44 is coinherited in trans with
HLA-B8. Whereas the alloreactive LC13 TCR docked at the C terminus of
HLA-B8-FLRGRAYGL, the CF34 TCR docked at the N terminus of HLA-B8-FLRGRAYGL,
which coincided with a polymorphic region between HLA-B8 and HLA-B44. The
markedly contrasting footprints of the LC13 and CF34 TCRs provided a portrait of
how self-tolerance shapes the specificity of TCRs selected into the immune
repertoire.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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A.K.Sewell
(2012).
Why must T cells be cross-reactive?
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Nat Rev Immunol,
12,
669-677.
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D.K.Sethi,
D.A.Schubert,
A.K.Anders,
A.Heroux,
D.A.Bonsor,
C.P.Thomas,
E.J.Sundberg,
J.Pyrdol,
and
K.W.Wucherpfennig
(2011).
A highly tilted binding mode by a self-reactive T cell receptor results in altered engagement of peptide and MHC.
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J Exp Med,
208,
91.
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PDB code:
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S.Gras,
L.Kjer-Nielsen,
Z.Chen,
J.Rossjohn,
and
J.McCluskey
(2011).
The structural bases of direct T-cell allorecognition: implications for T-cell-mediated transplant rejection.
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Immunol Cell Biol,
89,
388-395.
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Y.Yin,
Y.Li,
M.C.Kerzic,
R.Martin,
and
R.A.Mariuzza
(2011).
Structure of a TCR with high affinity for self-antigen reveals basis for escape from negative selection.
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EMBO J,
30,
1137-1148.
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PDB code:
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S.Gras,
Z.Chen,
J.J.Miles,
Y.C.Liu,
M.J.Bell,
L.C.Sullivan,
L.Kjer-Nielsen,
R.M.Brennan,
J.M.Burrows,
M.A.Neller,
R.Khanna,
A.W.Purcell,
A.G.Brooks,
J.McCluskey,
J.Rossjohn,
and
S.R.Burrows
(2010).
Allelic polymorphism in the T cell receptor and its impact on immune responses.
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J Exp Med,
207,
1555-1567.
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PDB codes:
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X.C.Li,
and
M.Raghavan
(2010).
Structure and function of major histocompatibility complex class I antigens.
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Curr Opin Organ Transplant,
15,
499-504.
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D.M.Kranz
(2009).
Two mechanisms that account for major histocompatibility complex restriction of T cells.
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F1000 Biol Rep,
1,
0.
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J.J.van Rood,
and
M.Oudshoorn
(2009).
When selecting an HLA mismatched stem cell donor consider donor immune status.
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Curr Opin Immunol,
21,
538-543.
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K.W.Wucherpfennig,
M.J.Call,
L.Deng,
and
R.Mariuzza
(2009).
Structural alterations in peptide-MHC recognition by self-reactive T cell receptors.
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Curr Opin Immunol,
21,
590-595.
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W.A.Macdonald,
Z.Chen,
S.Gras,
J.K.Archbold,
F.E.Tynan,
C.S.Clements,
M.Bharadwaj,
L.Kjer-Nielsen,
P.M.Saunders,
M.C.Wilce,
F.Crawford,
B.Stadinsky,
D.Jackson,
A.G.Brooks,
A.W.Purcell,
J.W.Kappler,
S.R.Burrows,
J.Rossjohn,
and
J.McCluskey
(2009).
T cell allorecognition via molecular mimicry.
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Immunity,
31,
897-908.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
