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PDBsum entry 3kpp
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Immune system PDB id
3kpp

 

 

 

 

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Contents
Protein chains
276 a.a. *
99 a.a. *
Ligands
GLU-GLU-TYR-LEU-
GLN-ALA-PHE-THR-
TYR
PEG ×2
Waters ×497
* Residue conservation analysis
PDB id:
3kpp
Name: Immune system
Title: Crystal structure of hla b 4405 In complex with eeylqafty a self peptide from the abcd3 protein
Structure: Hla class i histocompatibility antigen, b-44 alpha chain. Chain: a. Fragment: unp residues 25-300. Synonym: mhc class i antigen b 44, Bw-44. Engineered: yes. Beta-2-microglobulin. Chain: b. Synonym: beta-2-microglobulin form pi 5.3. Engineered: yes.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hla-b, hlab. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: b2m, cdabp0092, hdcma22p. Synthetic: yes
Resolution:
1.90Å     R-factor:   0.188     R-free:   0.230
Authors: W.A.Macdonald,Z.Chen,S.Gras,J.K.Archbold,F.E.Tynan,C.S.Clements, M.Bharadwaj,L.Kjer-Nielsen,P.M.Saunders,M.C.Wilce,F.Crawford, B.Stadinsky,D.Jackson,A.G.Brooks,A.W.Purcell,J.W.Kappler, S.R.Burrows,J.Rossjohn,J.Mccluskey
Key ref: W.A.Macdonald et al. (2009). T cell allorecognition via molecular mimicry. Immunity, 31, 897-908. PubMed id: 20064448
Date:
16-Nov-09     Release date:   22-Dec-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P01889  (1B07_HUMAN) -  HLA class I histocompatibility antigen, B alpha chain from Homo sapiens
Seq:
Struc: 		362 a.a.
276 a.a.*
Protein chain
Pfam   ArchSchema ?
P61769  (B2MG_HUMAN) -  Beta-2-microglobulin from Homo sapiens
Seq:
Struc: 		119 a.a.
99 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 30 residue positions (black crosses)

 

 
Immunity 31:897-908 (2009)
PubMed id: 20064448  
 
 
T cell allorecognition via molecular mimicry.
W.A.Macdonald, Z.Chen, S.Gras, J.K.Archbold, F.E.Tynan, C.S.Clements, M.Bharadwaj, L.Kjer-Nielsen, P.M.Saunders, M.C.Wilce, F.Crawford, B.Stadinsky, D.Jackson, A.G.Brooks, A.W.Purcell, J.W.Kappler, S.R.Burrows, J.Rossjohn, J.McCluskey.
 
  ABSTRACT  
 
T cells often alloreact with foreign human leukocyte antigens (HLA). Here we showed the LC13 T cell receptor (TCR), selected for recognition on self-HLA-B( *)0801 bound to a viral peptide, alloreacts with B44 allotypes (HLA-B( *)4402 and HLA-B( *)4405) bound to two different allopeptides. Despite extensive polymorphism between HLA-B( *)0801, HLA-B( *)4402, and HLA-B( *)4405 and the disparate sequences of the viral and allopeptides, the LC13 TCR engaged these peptide-HLA (pHLA) complexes identically, accommodating mimicry of the viral peptide by the allopeptide. The viral and allopeptides adopted similar conformations only after TCR ligation, revealing an induced-fit mechanism of molecular mimicry. The LC13 T cells did not alloreact against HLA-B( *)4403, and the single residue polymorphism between HLA-B( *)4402 and HLA-B( *)4403 affected the plasticity of the allopeptide, revealing that molecular mimicry was associated with TCR specificity. Accordingly, molecular mimicry that is HLA and peptide dependent is a mechanism for human T cell alloreactivity between disparate cognate and allogeneic pHLA complexes.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
23154222 J.Rossjohn, D.G.Pellicci, O.Patel, L.Gapin, and D.I.Godfrey (2012).
Recognition of CD1d-restricted antigens by natural killer T cells.
  Nat Rev Immunol, 12, 845-857.  
22722860 P.T.Illing, J.P.Vivian, N.L.Dudek, L.Kostenko, Z.Chen, M.Bharadwaj, J.J.Miles, L.Kjer-Nielsen, S.Gras, N.A.Williamson, S.R.Burrows, A.W.Purcell, J.Rossjohn, and J.McCluskey (2012).
Immune self-reactivity triggered by drug-modified HLA-peptide repertoire.
  Nature, 486, 554-558.
PDB codes: 3vri 3vrj
21321209 G.P.Morris, P.P.Ni, and P.M.Allen (2011).
Alloreactivity is limited by the endogenous peptide repertoire.
  Proc Natl Acad Sci U S A, 108, 3695-3700.  
  21446969 I.R.Ferrer, K.Araki, and M.L.Ford (2011).
Paradoxical aspects of rapamycin immunobiology in transplantation.
  Am J Transplant, 11, 654-659.  
21364947 J.M.Khan, and S.Ranganathan (2011).
Understanding TR Binding to pMHC Complexes: How Does a TR Scan Many pMHC Complexes yet Preferentially Bind to One.
  PLoS One, 6, e17194.  
21242884 L.J.D'orsogna, D.L.Roelen, E.M.van der Meer-Prins, P.van der Pol, M.E.Franke-van Dijk, M.Eikmans, J.Anholts, J.Rossjohn, J.McCluskey, A.Mulder, C.van Kooten, I.I.Doxiadis, and F.H.Claas (2011).
Tissue Specificity of Cross-Reactive Allogeneic Responses by EBV EBNA3A-Specific Memory T Cells.
  Transplantation, 91, 494-500.  
21283063 L.J.D'orsogna, N.M.van Besouw, E.M.van der Meer-Prins, P.van der Pol, M.Franke-van Dijk, Y.M.Zoet, A.van der Slik, W.Weimar, C.van Kooten, A.Mulder, D.L.Roelen, I.I.Doxiadis, and F.H.Claas (2011).
Vaccine-Induced Allo-HLA-Reactive Memory T Cells in a Kidney Transplantation Candidate.
  Transplantation, 91, 645-651.  
  21301478 S.Gras, L.Kjer-Nielsen, Z.Chen, J.Rossjohn, and J.McCluskey (2011).
The structural bases of direct T-cell allorecognition: implications for T-cell-mediated transplant rejection.
  Immunol Cell Biol, 89, 388-395.  
21092021 S.Y.Ko, H.B.Oh, Y.S.Heo, J.H.Jun, and O.J.Kwon (2011).
B*39:60, a novel HLA-B*39 allele identified by sequence-based typing.
  Tissue Antigens, 77, 155-156.  
20616723 C.T.Spencer, P.Gilchuk, S.M.Dragovic, and S.Joyce (2010).
Minor histocompatibility antigens: presentation principles, recognition logic and the potential for a healing hand.
  Curr Opin Organ Transplant, 15, 512-525.  
20639478 D.K.Cole, E.S.Edwards, K.K.Wynn, M.Clement, J.J.Miles, K.Ladell, J.Ekeruche, E.Gostick, K.J.Adams, A.Skowera, M.Peakman, L.Wooldridge, D.A.Price, and A.K.Sewell (2010).
Modification of MHC anchor residues generates heteroclitic peptides that alter TCR binding and T cell recognition.
  J Immunol, 185, 2600-2610.  
21173256 H.N.Eisen, and A.K.Chakraborty (2010).
Evolving concepts of specificity in immune reactions.
  Proc Natl Acad Sci U S A, 107, 22373-22380.  
20616729 M.L.Ford, and C.P.Larsen (2010).
Overcoming the memory barrier in tolerance induction: molecular mimicry and functional heterogeneity among pathogen-specific T-cell populations.
  Curr Opin Organ Transplant, 15, 405-410.  
21042237 N.A.Mifsud, T.H.Nguyen, B.D.Tait, and T.C.Kotsimbos (2010).
Quantitative and functional diversity of cross-reactive EBV-specific CD8+ T cells in a longitudinal study cohort of lung transplant recipients.
  Transplantation, 90, 1439-1449.  
20616724 S.A.Leddon, and A.J.Sant (2010).
Generation of MHC class II-peptide ligands for CD4 T-cell allorecognition of MHC class II molecules.
  Curr Opin Organ Transplant, 15, 505-511.  
20064442 Y.Yin, and R.A.Mariuzza (2009).
The multiple mechanisms of T cell receptor cross-reactivity.
  Immunity, 31, 849-851.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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