PDBsum entry 3ewb

Transferase

PDB id: 3ewb

Contents

Protein chain

271 a.a. *

Waters ×109

* Residue conservation analysis

PDB id:

3ewb

Name:

Transferase

Title:

Crystal structure of n-terminal domain of putative 2-isopropylmalate synthase from listeria monocytogenes

Structure:

2-isopropylmalate synthase. Chain: x. Fragment: n-terminal domain (2-283). Synonym: alpha-isopropylmalate synthase, alpha-ipm synthetase. Engineered: yes

Source:

Listeria monocytogenes str. 4b f2365. Organism_taxid: 265669. Gene: leua, lmof2365_2010. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.196 R-free: 0.245

Authors:

U.A.Ramagopal,R.Toro,M.Gilmore,S.Hu,M.Maletic,L.Rodgers,S.K.Burley, S.C.Almo,New York Sgx Research Center For Structural Genomics (Nysgxrc)

Key ref:

U.A.Ramagopal et al. Crystal structure of n-Terminal domain of putative 2-Isopropylmalate synthase from listeria monocytogenes.. To be published, .

Date:

14-Oct-08 Release date: 23-Dec-08

Protein chain

Q71Y35  (LEU1_LISMF) -  2-isopropylmalate synthase from Listeria monocytogenes serotype 4b (strain F2365)

Seq: 512 a.a.

Struc: 271 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.3.13 - 2-isopropylmalate synthase.
• Pathway: Leucine Biosynthesis
• Reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H⁺
• Cofactor: K(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site