PDBsum entry 3de9

Metal binding protein

PDB id

3de9

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Contents

			Protein chain

					106 a.a. *

			Ligands

					HEM

			Metals

					_NI ×3

			Waters ×55

* Residue conservation analysis

PDB id:

3de9

Links

Name:

Metal binding protein

Title:

Crystal structure of a trimeric cytochrome cb562 assembly induced by nickel coordination

Structure:

Soluble cytochrome b562. Chain: a. Synonym: cytochrome b-562. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: cybc. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.04Å

R-factor:

0.222

R-free:

0.278

Authors:

E.N.Salgado,R.A.Lewis,A.L.Rheingold,F.A.Tezcan

Key ref:

E.N.Salgado et al. (2009). Control of protein oligomerization symmetry by metal coordination: C2 and C3 symmetrical assemblies through Cu(II) and Ni(II) coordination. Inorg Chem, 48, 2726-2728. PubMed id: 19267481

Date:

08-Jun-08

Release date:

21-Apr-09

PROCHECK

	Headers

	References

Protein chain

P0ABE7 (C562_ECOLX) - Soluble cytochrome b562 from Escherichia coli

Seq: Struc:					128 a.a. 106 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 5 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Inorg Chem 48:2726-2728 (2009)
PubMed id: 19267481

Control of protein oligomerization symmetry by metal coordination: C2 and C3 symmetrical assemblies through Cu(II) and Ni(II) coordination.
E.N.Salgado, R.A.Lewis, S.Mossin, A.L.Rheingold, F.A.Tezcan.

ABSTRACT

We describe the metal-dependent self-assembly of symmetrical protein homooligomers from protein building blocks that feature appropriately engineered metal-chelating motifs on their surfaces. Crystallographic studies indicate that the same four-helix-bundle protein construct, MBPC-1, can self-assemble into C(2) and C(3) symmetrical assemblies dictated by Cu(II) and Ni(II) coordination, respectively. The symmetry inherent in metal coordination can thus be directly applied to biological self-assembly.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20189099

B.Höcker (2010).
Metals make proteins stick.

Chem Biol, 17, 103-104.

20192262

E.N.Salgado, R.J.Radford, and F.A.Tezcan (2010).
Metal-directed protein self-assembly.

Acc Chem Res, 43, 661-672.

20080561

E.N.Salgado, X.I.Ambroggio, J.D.Brodin, R.A.Lewis, B.Kuhlman, and F.A.Tezcan (2010).
Metal templated design of protein interfaces.

Proc Natl Acad Sci U S A, 107, 1827-1832.

PDB codes:

3hni 3hnj 3hnk 3hnl

20445239

M.Rajavel, and B.Gopal (2010).
Analysis of multiple crystal forms of Bacillus subtilis BacB suggests a role for a metal ion as a nucleant for crystallization.

Acta Crystallogr D Biol Crystallogr, 66, 635-639.

PDB code:

3h9a

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.