PDBsum entry 3d0r

Transferase

PDB id

3d0r

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Contents

			Protein chains

					378 a.a. *

			Ligands

					PE4

			Waters ×579

* Residue conservation analysis

PDB id:

3d0r

Links

Name:

Transferase

Title:

Crystal structure of calg3 from micromonospora echinospora determined in space group p2(1)

Structure:

Protein calg3. Chain: a, b. Engineered: yes

Source:

Micromonospora echinospora. Organism_taxid: 1877. Strain: ll6600. Gene: calg3. Expressed in: escherichia coli.

Resolution:

1.90Å

R-factor:

0.162

R-free:

0.212

Authors:

E.Bitto,C.A.Bingman,G.E.Wesenberg,G.N.Phillips Jr.

Key ref:

C.Zhang et al. (2008). Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis. Chem Biol, 15, 842-853. PubMed id: 18721755

Date:

02-May-08

Release date:

24-Jun-08

PROCHECK

	Headers

	References

Protein chains

Q8KND7 (Q8KND7_MICEC) - CalG3 from Micromonospora echinospora

Seq: Struc:					376 a.a. 378 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

	Chem Biol 15:842-853 (2008)
PubMed id: 18721755

Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis.
C.Zhang, E.Bitto, R.D.Goff, S.Singh, C.A.Bingman, B.R.Griffith, C.Albermann, G.N.Phillips, J.S.Thorson.

ABSTRACT

The enediyne antibiotic calicheamicin (CLM) gamma(1)(I) is a prominent antitumor agent that is targeted to DNA by a novel aryltetrasaccharide comprised of an aromatic unit and four unusual carbohydrates. Herein we report the heterologous expression and the biochemical characterization of the two "internal" glycosyltransferases CalG3 and CalG2 and the structural elucidation of an enediyne glycosyltransferase (CalG3). In conjunction with the previous characterization of the "external" CLM GTs CalG1 and CalG4, this study completes the functional assignment of all four CLM GTs, extends the utility of enediyne GT-catalyzed reaction reversibility, and presents conclusive evidence of a sequential glycosylation pathway in CLM biosynthesis. This work also reveals the common GT-B structural fold can now be extended to include enediyne GTs.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20418426

L.Kaysser, E.Wemakor, S.Siebenberg, J.A.Salas, J.K.Sohng, B.Kammerer, and B.Gust (2010).
Formation and attachment of the deoxysugar moiety and assembly of the gene cluster for caprazamycin biosynthesis.

Appl Environ Microbiol, 76, 4008-4018.

20336235

Z.X.Liang (2010).
Complexity and simplicity in the biosynthesis of enediyne natural products.

Nat Prod Rep, 27, 499-528.

19233921

A.Ramos, C.Olano, A.F.Braña, C.Méndez, and J.A.Salas (2009).
Modulation of deoxysugar transfer by the elloramycin glycosyltransferase ElmGT through site-directed mutagenesis.

J Bacteriol, 191, 2871-2875.

19290519

D.Simkhada, T.J.Oh, B.B.Pageni, H.C.Lee, K.Liou, and J.K.Sohng (2009).
Characterization of CalS9 in the biosynthesis of UDP-xylose and the production of xylosyl-attached hybrid compound.

Appl Microbiol Biotechnol, 83, 885-895.

18798210

C.Zhang, R.Moretti, J.Jiang, and J.S.Thorson (2008).
The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.

Chembiochem, 9, 2506-2514.

18678278

G.J.Williams, R.W.Gantt, and J.S.Thorson (2008).
The impact of enzyme engineering upon natural product glycodiversification.

Curr Opin Chem Biol, 12, 556-564.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.