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PDBsum entry 3d0r
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References listed in PDB file
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Key reference
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Title
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Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis.
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Authors
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C.Zhang,
E.Bitto,
R.D.Goff,
S.Singh,
C.A.Bingman,
B.R.Griffith,
C.Albermann,
G.N.Phillips,
J.S.Thorson.
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Ref.
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Chem Biol, 2008,
15,
842-853.
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PubMed id
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Abstract
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The enediyne antibiotic calicheamicin (CLM) gamma(1)(I) is a prominent antitumor
agent that is targeted to DNA by a novel aryltetrasaccharide comprised of an
aromatic unit and four unusual carbohydrates. Herein we report the heterologous
expression and the biochemical characterization of the two "internal"
glycosyltransferases CalG3 and CalG2 and the structural elucidation of an
enediyne glycosyltransferase (CalG3). In conjunction with the previous
characterization of the "external" CLM GTs CalG1 and CalG4, this study completes
the functional assignment of all four CLM GTs, extends the utility of enediyne
GT-catalyzed reaction reversibility, and presents conclusive evidence of a
sequential glycosylation pathway in CLM biosynthesis. This work also reveals the
common GT-B structural fold can now be extended to include enediyne GTs.
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