PDBsum entry 3ba8

Ligase

PDB id

3ba8

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Contents

			Protein chain

					313 a.a. *

			Ligands

					SO4 ×3


					NMN


					3B8

			Waters ×284

* Residue conservation analysis

PDB id:

3ba8

Links
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Name:

Ligase

Title:

Structural basis for the inhibition of bacterial NAD+ dependent DNA ligase

Structure:

DNA ligase. Chain: a. Fragment: adenylation domain. Engineered: yes

Source:

Enterococcus faecalis. Streptococcus faecalis. Organism_taxid: 1351. Gene: liga. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.90Å

R-factor:

0.193

R-free:

0.223

Authors:

C.Pinko

Key ref:

C.Pinko et al. Structural basis for the inhibition of bacterial NAD+ dependent DNA ligase. To be published, .

Date:

07-Nov-07

Release date:

11-Nov-08

PROCHECK

	Headers

	References

Protein chain

Q837V6 (DNLJ_ENTFA) - DNA ligase from Enterococcus faecalis (strain ATCC 700802 / V583)

Seq: Struc:

Seq: Struc:					676 a.a. 313 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.6.5.1.2 - Dna ligase (NAD(+)).

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

NAD⁺ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta- nicotinamide D-nucleotide

NAD(+)

(deoxyribonucleotide)n-3'-hydroxyl

5'-phospho- (deoxyribonucleotide)m

(deoxyribonucleotide)n+m

AMP
Bound ligand (Het Group name = NMN)
matches with 73.08% similarity

beta- nicotinamide D-nucleotide

Molecule diagrams generated from .mol files obtained from the KEGG ftp site