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PDBsum entry 3azk
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protein dna_rna metals Protein-protein interface(s) links
Structural protein/DNA PDB id
3azk

 

 

 

 

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Contents
Protein chains
97 a.a.
78 a.a.
108 a.a.
95 a.a.
84 a.a.
DNA/RNA
Metals
_CL ×4
_MN ×11
PDB id:
3azk
Name: Structural protein/DNA
Title: Crystal structure of human nucleosome core particle containing h4k59q mutation
Structure: Histone h3.1. Chain: a, e. Synonym: histone h3/a, histone h3/b, histone h3/c, histone h3/d, histone h3/f, histone h3/h, histone h3/i, histone h3/j, histone h3/k, histone h3/l. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Resolution:
3.20Å     R-factor:   0.200     R-free:   0.258
Authors: W.Iwasaki,H.Tachiwana,K.Kawaguchi,T.Shibata,W.Kagawa,H.Kurumizaka
Key ref: W.Iwasaki et al. (2011). Comprehensive structural analysis of mutant nucleosomes containing lysine to glutamine (KQ) substitutions in the H3 and H4 histone-fold domains. Biochemistry, 50, 7822-7832. PubMed id: 21812398
Date:
25-May-11     Release date:   21-Sep-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens
Seq:
Struc: 		136 a.a.
97 a.a.
Protein chain
Pfam   ArchSchema ?
P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens
Seq:
Struc: 		103 a.a.
78 a.a.*
Protein chains
Pfam   ArchSchema ?
P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens
Seq:
Struc: 		130 a.a.
108 a.a.
Protein chains
Pfam   ArchSchema ?
P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens
Seq:
Struc: 		126 a.a.
95 a.a.
Protein chain
Pfam   ArchSchema ?
P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens
Seq:
Struc: 		103 a.a.
84 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains
  A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 145 bases
  T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G-G- 145 bases

 

 
Biochemistry 50:7822-7832 (2011)
PubMed id: 21812398  
 
 
Comprehensive structural analysis of mutant nucleosomes containing lysine to glutamine (KQ) substitutions in the H3 and H4 histone-fold domains.
W.Iwasaki, H.Tachiwana, K.Kawaguchi, T.Shibata, W.Kagawa, H.Kurumizaka.
 
  ABSTRACT  
 
No abstract given.

 

 

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