PDBsum entry 3ajh

Oxidoreductase

PDB id

3ajh

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Contents

			Protein chains

					240 a.a.

			Ligands

					BL3 ×2

			Waters ×134

PDB id:

3ajh

Links

Name:

Oxidoreductase

Title:

Crystal structure of pcya v225d-biliverdin xiii alpha complex

Structure:

Phycocyanobilin:ferredoxin oxidoreductase. Chain: a, b. Fragment: v225d. Engineered: yes

Source:

Synechocystis. Organism_taxid: 1148. Strain: pcc 6803. Gene: pcya, slr0116. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.25Å

R-factor:

0.251

R-free:

0.289

Authors:

K.Wada,Y.Hagiwara,K.Fukuyama

Key ref:

K.Wada et al. (2010). One residue substitution in PcyA leads to unexpected changes in tetrapyrrole substrate binding. Biochem Biophys Res Commun, 402, 373-377. PubMed id: 20946883

Date:

05-Jun-10

Release date:

16-Mar-11

PROCHECK

	Headers

	References

Protein chains

Q55891 (PCYA_SYNY3) - Phycocyanobilin:ferredoxin oxidoreductase from Synechocystis sp. (strain PCC 6803 / Kazusa)

Seq: Struc:					248 a.a. 240 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.1.3.7.5 - phycocyanobilin:ferredoxin oxidoreductase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Biliverdin metabolism

Reaction:

(2R,3Z)-phycocyanobilin + 4 oxidized [2Fe-2S]-[ferredoxin] = biliverdin IXalpha + 4 reduced [2Fe-2S]-[ferredoxin] + 4 H⁺

(3Z)-phycocyanobilin	+	4 × oxidized ferredoxin	=	biliverdin IX-alpha	+	4 × reduced ferredoxin

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Biochem Biophys Res Commun 402:373-377 (2010)
PubMed id: 20946883

One residue substitution in PcyA leads to unexpected changes in tetrapyrrole substrate binding.
K.Wada, Y.Hagiwara, Y.Yutani, K.Fukuyama.

ABSTRACT

No abstract given.