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PDBsum entry 3abh
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Contents |
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* Residue conservation analysis
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PDB id:
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Endocytosis
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Title:
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Crystal structure of the efc/f-bar domain of human pacsin2/syndapin ii (2.0 a)
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Structure:
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Protein kinasE C and casein kinase substrate in neurons protein 2. Chain: a, b. Fragment: efc/f-bar domain. Synonym: pacsin2. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: pacsin2. Expressed in: cell free system.
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Resolution:
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2.00Å
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R-factor:
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0.234
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R-free:
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0.271
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Authors:
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A.Shimada,M.Shirouzu,K.Hanawa-Suetsugu,T.Terada,T.Umehara,S.Suetsugu, M.Yamamoto,S.Yokoyama
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Key ref:
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A.Shimada
et al.
(2010).
Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II.
Febs Lett,
584,
1111-1118.
PubMed id:
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Date:
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11-Dec-09
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Release date:
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14-Apr-10
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PROCHECK
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Headers
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References
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Q9UNF0
(PACN2_HUMAN) -
Protein kinase C and casein kinase substrate in neurons protein 2 from Homo sapiens
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Seq:
Struc:
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486 a.a.
288 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Febs Lett
584:1111-1118
(2010)
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PubMed id:
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Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II.
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A.Shimada,
K.Takano,
M.Shirouzu,
K.Hanawa-Suetsugu,
T.Terada,
K.Toyooka,
T.Umehara,
M.Yamamoto,
S.Yokoyama,
S.Suetsugu.
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ABSTRACT
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The extended Fes-CIP4 homology (EFC)/FCH-BAR (F-BAR) domain tubulates membranes.
Overexpression of the pacsin2 EFC/F-BAR domain resulted in tubular localization
inside cells and deformed liposomes into tubules in vitro. We found that
overexpression of the pacsin2 EFC/F-BAR domain induced cellular microspikes,
with the pacsin2 EFC/F-BAR domain concentrated at the neck. The hydrophobic
loops and the basic amino-acid residues on the concave surface of the pacsin2
EFC/F-BAR domain are essential for both the microspike formation and tubulation.
Since the curvature of the neck of the microspike and that of the tubulation
share similar geometry, the pacsin2 EFC/F-BAR domain is considered to facilitate
both microspike formation and tubulation.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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D.Ruffell
(2011).
Featuring… Shiro Suetsugu Winner of the 2011 FEBS Letters Young Group Leader Award.
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FEBS Lett,
585,
1504-1505.
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R.Zaidel-Bar,
M.J.Joyce,
A.M.Lynch,
K.Witte,
A.Audhya,
and
J.Hardin
(2010).
The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogenesis.
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J Cell Biol,
191,
761-769.
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S.Suetsugu
(2010).
The proposed functions of membrane curvatures mediated by the BAR domain superfamily proteins.
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J Biochem,
148,
1.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
