 |
PDBsum entry 3abh
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Mapping of the basic amino-Acid residues responsible for tubulation and cellular protrusion by the efc/f-Bar domain of pacsin2/syndapin ii.
|
|
|
Authors
|
|
A.Shimada,
K.Takano,
M.Shirouzu,
K.Hanawa-Suetsugu,
T.Terada,
K.Toyooka,
T.Umehara,
M.Yamamoto,
S.Yokoyama,
S.Suetsugu.
|
|
|
Ref.
|
|
Febs Lett, 2010,
584,
1111-1118.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The extended Fes-CIP4 homology (EFC)/FCH-BAR (F-BAR) domain tubulates membranes.
Overexpression of the pacsin2 EFC/F-BAR domain resulted in tubular localization
inside cells and deformed liposomes into tubules in vitro. We found that
overexpression of the pacsin2 EFC/F-BAR domain induced cellular microspikes,
with the pacsin2 EFC/F-BAR domain concentrated at the neck. The hydrophobic
loops and the basic amino-acid residues on the concave surface of the pacsin2
EFC/F-BAR domain are essential for both the microspike formation and tubulation.
Since the curvature of the neck of the microspike and that of the tubulation
share similar geometry, the pacsin2 EFC/F-BAR domain is considered to facilitate
both microspike formation and tubulation.
|
|
|
|
|
|
|
