PDBsum entry 3v6c

Hydrolase/signaling protein

PDB id: 3v6c

Contents

Protein chains

327 a.a.

81 a.a.

Ligands

GOL

Metals

_ZN

_CL ×2

Waters ×291

PDB id:

3v6c

Name:

Hydrolase/signaling protein

Title:

Crystal structure of usp2 in complex with mutated ubiquitin

Structure:

Ubiquitin carboxyl-terminal hydrolase 2. Chain: a. Synonym: 41 kda ubiquitin-specific protease, deubiquitinating enzyme 2, ubiquitin thiolesterase 2, ubiquitin-specific-processing protease 2. Engineered: yes. Ubiquitin. Chain: b. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ubp41, usp02.258.605, usp2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: ubc.

Resolution:

1.70Å R-factor: 0.178 R-free: 0.220

Authors:

M.Neculai,A.Ernst,S.Sidhu,C.H.Arrowsmith,A.M.Edwards,C.Bountra, J.Weigelt,S.Dhe-Paganon,Structural Genomics Consortium (Sgc)

Key ref:

M.Neculai et al. Crystal structure of usp2 in complex with mutated ubi. To be published, .

Date:

19-Dec-11 Release date: 19-Dec-12

Protein chain

O75604  (UBP2_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 2 from Homo sapiens

Seq: 605 a.a.

Struc: 327 a.a.

Protein chain

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 81 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
• Enzyme class 3: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.