 |
PDBsum entry 3v5m
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase
|
|
|
Title:
|
|
Crystal structure of m69v mutant of shv beta-lactamase
|
|
Structure:
|
|
Beta-lactamase. Chain: a. Fragment: shv-1 beta-lactamase. Synonym: beta-lactamase shv-1, beta-lactamase bla-shv-1, beta- lactamase blashv-1, bla-shv type 1, broad-spectrum beta-lactamase shv-1, extended-spectrum beta-lactamase shv-11, shv type beta lactamase, shv type extended spectrum beta-lactamase, shv-1 beta- lactamase. Engineered: yes.
|
|
Source:
|
|
Klebsiella pneumoniae. Organism_taxid: 573. Gene: blashv-1, blactx-m, blashv, blashv-11, blashv1, shv, shv-1, shv-1, shv-1b-a. Expressed in: escherichia coli. Expression_system_taxid: 316385.
|
|
Resolution:
|
|
|
1.30Å
|
R-factor:
|
0.165
|
R-free:
|
0.186
|
|
|
Authors:
|
|
F.Van Den Akker,E.A.Rodkey
|
|
Key ref:
|
|
W.Ke
et al.
(2012).
The importance of the trans-enamine intermediate as a β-lactamase inhibition strategy probed in inhibitor-resistant SHV β-lactamase variants.
Chemmedchem,
7,
1002-1008.
PubMed id:
|
|
|
Date:
|
|
|
16-Dec-11
|
Release date:
|
08-Aug-12
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P0AD64
(BLA1_KLEPN) -
Beta-lactamase SHV-1 from Klebsiella pneumoniae
|
|
|
|
Seq:
Struc:
|
|
|
|
286 a.a.
265 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Chemmedchem
7:1002-1008
(2012)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
The importance of the trans-enamine intermediate as a β-lactamase inhibition strategy probed in inhibitor-resistant SHV β-lactamase variants.
|
|
W.Ke,
E.A.Rodkey,
J.M.Sampson,
M.J.Skalweit,
A.Sheri,
S.R.Pagadala,
M.D.Nottingham,
J.D.Buynak,
R.A.Bonomo,
F.van den Akker.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
