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PDBsum entry 3blb
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Contents |
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* Residue conservation analysis
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PDB id:
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Hydrolase
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Title:
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Crystal structure of golgi mannosidase ii in complex with swainsonine at 1.3 angstrom resolution
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Structure:
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Alpha-mannosidase 2. Chain: a. Fragment: catalytic domain: residues 76-1108. Synonym: alpha-mannosidase ii, mannosyl-oligosaccharide 1,3-1,6- alpha-mannosidase, man ii, golgi alpha-mannosidase ii, aman ii. Engineered: yes
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Source:
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Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Strain: berkeley. Tissue: head. Gene: alpha-man-ii, gmii, cg18802. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.
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Resolution:
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1.30Å
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R-factor:
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0.166
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R-free:
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0.185
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Authors:
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D.A.Kuntz,D.R.Rose
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Key ref:
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D.A.Kuntz
and
d.r.rose
Golgi mannosidase ii in complex with swainsonine at 1.3 angstrom..
To be published,
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Date:
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10-Dec-07
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Release date:
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08-Jan-08
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PROCHECK
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Headers
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References
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Q24451
(MAN2_DROME) -
Alpha-mannosidase 2 from Drosophila melanogaster
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Seq:
Struc:
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1108 a.a.
1014 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.2.1.114
- mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase.
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Pathway:
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Mannosyl-glycoprotein N-acetylglucosaminyltransferases
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Reaction:
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N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha- D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 H2O = 2 alpha-D-mannopyranose + an N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]- beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl- [protein]
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Links
