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PDBsum entry 2xg0
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protein dna_rna Protein-protein interface(s) links
Ribosome PDB id
2xg0

 

 

 

 

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Contents
Protein chains
84 a.a. *
94 a.a. *
71 a.a. *
60 a.a. *
56 a.a. *
59 a.a. *
45 a.a. *
49 a.a. *
64 a.a. *
36 a.a. *
191 a.a. *
272 a.a. *
205 a.a. *
208 a.a. *
181 a.a. *
165 a.a. *
145 a.a. *
130 a.a. *
139 a.a. *
122 a.a. *
146 a.a. *
141 a.a. *
117 a.a. *
99 a.a. *
138 a.a. *
117 a.a. *
101 a.a. *
113 a.a. *
93 a.a. *
101 a.a. *
187 a.a. *
DNA/RNA
* Residue conservation analysis
Obsolete entry
PDB id:
2xg0
Name: Ribosome
Title: Structure of cytotoxic domain of colicin e3 bound to the 70s ribosome (part 2 of 4)
Structure: 50s ribosomal protein l27. Chain: 0. 50s ribosomal protein l28. Chain: 1. 50s ribosomal protein l29. Chain: 2. 50s ribosomal protein l30. Chain: 3. 50s ribosomal protein l31.
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Atcc: 27634. Atcc: 27634
Resolution:
3.20Å     R-factor:   0.228     R-free:   0.270
Authors: C.L.Ng,K.Lang,N.A.G.Meenan,A.Sharma,A.C.Kelley,C.Kleanthous, V.Ramakrishnan
Key ref: C.L.Ng et al. (2010). Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3. Nat Struct Biol, 17, 1241-1246. PubMed id: 20852642
Date:
30-May-10     Release date:   22-Sep-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P60493  (RL27_THET8) -  50S ribosomal protein L27 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		85 a.a.
84 a.a.
Protein chain
Pfam   ArchSchema ?
P60494  (RL28_THET8) -  50S ribosomal protein L28 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		98 a.a.
94 a.a.*
Protein chain
Pfam   ArchSchema ?
Q5SHP6  (RL29_THET8) -  50S ribosomal protein L29 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		72 a.a.
71 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHQ6  (RL30_THET8) -  50S ribosomal protein L30 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		60 a.a.
60 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SJE1  (RL31_THET8) -  50S ribosomal protein L31 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		71 a.a.
56 a.a.
Protein chain
Pfam   ArchSchema ?
P80339  (RL32_THET8) -  50S ribosomal protein L32 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		60 a.a.
59 a.a.
Protein chain
Pfam   ArchSchema ?
P35871  (RL33_THET8) -  50S ribosomal protein L33 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		54 a.a.
45 a.a.
Protein chain
Pfam   ArchSchema ?
P80340  (RL34_THET8) -  50S ribosomal protein L34 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		49 a.a.
49 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SKU1  (RL35_THET8) -  50S ribosomal protein L35 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		65 a.a.
64 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHR2  (RL36_THET8) -  50S ribosomal protein L36 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		37 a.a.
36 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SLP7  (RL1_THET8) -  50S ribosomal protein L1 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		229 a.a.
191 a.a.
Protein chain
Pfam   ArchSchema ?
P60405  (RL2_THET8) -  50S ribosomal protein L2 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		276 a.a.
272 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHN8  (RL3_THET8) -  50S ribosomal protein L3 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		206 a.a.
205 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHN9  (RL4_THET8) -  50S ribosomal protein L4 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		210 a.a.
208 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHQ0  (RL5_THET8) -  50S ribosomal protein L5 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		182 a.a.
181 a.a.
Protein chain
Q5SHQ3  (RL6_THET8) - 
Protein chain
Pfam   ArchSchema ?
Q5SLQ1  (RL9_THET8) -  50S ribosomal protein L9 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		148 a.a.
145 a.a.
Protein chain
No UniProt id for this chain
Struc: 130 a.a.
Protein chain
Pfam   ArchSchema ?
P60488  (RL13_THET8) -  50S ribosomal protein L13 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		140 a.a.
139 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHP8  (RL14_THET8) -  50S ribosomal protein L14 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		122 a.a.
122 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHQ7  (RL15_THET8) -  50S ribosomal protein L15 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		150 a.a.
146 a.a.
Protein chain
Pfam   ArchSchema ?
P60489  (RL16_THET8) -  50S ribosomal protein L16 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		141 a.a.
141 a.a.
Protein chain
Pfam   ArchSchema ?
Q9Z9H5  (RL17_THET8) -  50S ribosomal protein L17 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		118 a.a.
117 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHQ4  (RL18_THET8) -  50S ribosomal protein L18 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		112 a.a.
99 a.a.
Protein chain
Pfam   ArchSchema ?
P60490  (RL19_THET8) -  50S ribosomal protein L19 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		146 a.a.
138 a.a.
Protein chain
Pfam   ArchSchema ?
P60491  (RL20_THET8) -  50S ribosomal protein L20 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		118 a.a.
117 a.a.
Protein chain
Pfam   ArchSchema ?
P60492  (RL21_THET8) -  50S ribosomal protein L21 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		101 a.a.
101 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHP3  (RL22_THET8) -  50S ribosomal protein L22 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		113 a.a.
113 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHP0  (RL23_THET8) -  50S ribosomal protein L23 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		96 a.a.
93 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHP9  (RL24_THET8) -  50S ribosomal protein L24 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		110 a.a.
101 a.a.
Protein chain
Pfam   ArchSchema ?
Q5SHZ1  (RL25_THET8) -  50S ribosomal protein L25 from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Seq:
Struc: 		206 a.a.
187 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains
  A-G-A-U-G-G-U-A-A-G-G-G-C-C-C-A-C-G-G-U-G-G-A-U-G-C-C-U-C-G-G-C-A-C-C-C-G-A-G- ... 2807 bases
  U-C-C-C-C-C-G-U-G-C-C-C-A-U-A-G-C-G-G-C-G-U-G-G-A-A-C-C-A-C-C-C-G-U-U-C-C-C-A- 119 bases

 

 
Nat Struct Biol 17:1241-1246 (2010)
PubMed id: 20852642  
 
 
Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3.
C.L.Ng, K.Lang, N.A.Meenan, A.Sharma, A.C.Kelley, C.Kleanthous, V.Ramakrishnan.
 
  ABSTRACT  
 
No abstract given.

 

 

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