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PDBsum entry 2xfh
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protein ligands links
Oxidoreductase PDB id
2xfh

 

 

 

 

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Contents
Protein chain
394 a.a. *
Ligands
HEM-CL6
CL6
DMS
Waters ×388
* Residue conservation analysis
PDB id:
2xfh
Name: Oxidoreductase
Title: Structure of cytochrome p450 eryk cocrystallized with inhibitor clotrimazole.
Structure: Erythromycin b/d c-12 hydroxylase. Chain: a. Synonym: cytocrome p450 cyp113a1, cytochrome p450 113a1. Engineered: yes. Mutation: yes
Source: Saccharopolyspora erythraea. Organism_taxid: 1836. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.90Å     R-factor:   0.184     R-free:   0.231
Authors: C.Savino,L.C.Montemiglio,S.Gianni,B.Vallone
Key ref: L.C.Montemiglio et al. (2010). Azole drugs trap cytochrome P450 EryK in alternative conformational states. Biochemistry, 49, 9199-9206. PubMed id: 20845962
Date:
24-May-10     Release date:   29-Sep-10    
Supersedes: 2vrv
PROCHECK
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 Headers
 References

Protein chain
P48635  (ERYK_SACEN) -  Erythromycin C-12 hydroxylase from Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Seq:
Struc: 		397 a.a.
394 a.a.
Key:    Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.1.14.13.154  - erythromycin 12 hydroxylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: erythromycin D + NADPH + O2 + H+ = erythromycin C + NADP+ + H2O
erythromycin D
 + NADPH
 + O2
 + H(+)
 = erythromycin C
 + NADP(+)
 + H2O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Biochemistry 49:9199-9206 (2010)
PubMed id: 20845962  
 
 
Azole drugs trap cytochrome P450 EryK in alternative conformational states.
L.C.Montemiglio, S.Gianni, B.Vallone, C.Savino.
 
  ABSTRACT  
 
No abstract given.

 

 

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