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UniProt functional annotation for P48635 | ||
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| UniProt code: P48635. |
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| Organism: | |
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338). |
| Taxonomy: | |
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Saccharopolyspora. |
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| Function: | |
Responsible for the C-12 hydroxylation of the macrolactone ring of erythromycin. Thus, EryK catalyzes the hydroxylation of erythromycin D (ErD) at the C-12 position to produce erythromycin C (ErC). Erythromycin B (ErB) is not a substrate for this enzyme. {ECO:0000269|PubMed:7849045, ECO:0000269|PubMed:8416893}. |
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| Catalytic activity: | |
Reaction=erythromycin D + H(+) + NADPH + O2 = erythromycin C + H2O + NADP(+); Xref=Rhea:RHEA:32631, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:63677, ChEBI:CHEBI:64258; EC=1.14.13.154; Evidence={ECO:0000269|PubMed:7849045}; |
| Cofactor: | |
Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000269|PubMed:19625248, ECO:0000269|PubMed:20845962, ECO:0000269|PubMed:7849045}; Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit. {ECO:0000269|PubMed:19625248, ECO:0000269|PubMed:20845962, ECO:0000269|PubMed:7849045}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=8.0 uM for erythromycin D {ECO:0000269|PubMed:7849045}; Note=kcat is 108 min(-1).; |
| Pathway: | |
Antibiotic biosynthesis; erythromycin biosynthesis. {ECO:0000269|PubMed:7849045}. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:19625248, ECO:0000269|PubMed:7849045}. |
| Disruption phenotype: | |
Cells lacking this gene no longer produce erythromycin A but accumulate the B and D forms of the antibiotic. {ECO:0000269|PubMed:8416893}. |
| Similarity: | |
Belongs to the cytochrome P450 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.