PDBsum entry 2xe4

Hydrolase/inhibitor

PDB id: 2xe4

Contents

Protein chain

721 a.a. *

Ligands

FC0-ARG-VAL-RGL

GOL ×10

PGR ×48

PGO

PO4 ×3

Metals

_CL ×4

_NA ×2

Waters ×721

* Residue conservation analysis

PDB id:

2xe4

Name:

Hydrolase/inhibitor

Title:

Structure of oligopeptidase b from leishmania major

Structure:

Oligopeptidase b. Chain: a. Synonym: family s9a-like protein, serine peptidase. Engineered: yes. Mutation: yes. Antipain. Chain: b

Source:

Leishmania major. Organism_taxid: 5664. Strain: friedlin. Expressed in: escherichia coli. Expression_system_taxid: 469008. Actinobacteria. Organism_taxid: 1760

Resolution:

1.65Å R-factor: 0.142 R-free: 0.178

Authors:

K.Mcluskey,N.G.Paterson,N.D.Bland,J.C.Mottram,N.W.Isaacs

Key ref:

K.McLuskey et al. (2010). Crystal structure of Leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor. J Biol Chem, 285, 39249-39259. PubMed id: 20926390

Date:

11-May-10 Release date: 06-Oct-10

Protein chain

Q4QHU7  (Q4QHU7_LEIMA) -  Prolyl endopeptidase from Leishmania major

Seq: 731 a.a.

Struc: 721 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.3.4.21.- - ?????
• Enzyme class 3: E.C.3.4.21.26 - prolyl oligopeptidase.
• Reaction: Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

J Biol Chem 285:39249-39259 (2010)

PubMed id: 20926390

Crystal structure of Leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor.

K.McLuskey, N.G.Paterson, N.D.Bland, N.W.Isaacs, J.C.Mottram.

ABSTRACT

No abstract given.