PDBsum entry 2w2j

Lyase

PDB id: 2w2j

Contents

Protein chain

268 a.a. *

Ligands

GOL ×6

UNX ×3

Metals

_CL ×3

Waters ×336

* Residue conservation analysis

PDB id:

2w2j

Name:

Lyase

Title:

Crystal structure of the human carbonic anhydrase related protein viii

Structure:

Carbonic anhydrase-related protein. Chain: a. Synonym: carp, carbonic anhydrase viii, ca-viii, ca8a. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.60Å R-factor: 0.201 R-free: 0.229

Authors:

A.Kramm,J.R.C.Muniz,S.S.Picaud,F.Von Delft,E.S.Pilka,W.W.Yue, O.N.F.King,G.Kochan,A.C.W.Pike,P.Filippakopoulos,C.Arrowsmith, M.Wikstrom,A.Edwards,C.Bountra,U.Oppermann

Key ref:

S.S.Picaud et al. (2009). Crystal structure of human carbonic anhydrase-related protein VIII reveals the basis for catalytic silencing. Proteins, 76, 507-511. PubMed id: 19360879 DOI: 10.1002/prot.22411

Date:

31-Oct-08 Release date: 03-Feb-09

Protein chain

P35219  (CAH8_HUMAN) -  Carbonic anhydrase-related protein from Homo sapiens

Seq: 290 a.a.

Struc: 268 a.a.

Enzyme reactions

• Enzyme class: E.C.4.2.1.1 - carbonic anhydrase.
• Reaction: hydrogencarbonate + H⁺ = CO2 + H2O
• Cofactor: Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1002/prot.22411

Proteins 76:507-511 (2009)

PubMed id: 19360879

Crystal structure of human carbonic anhydrase-related protein VIII reveals the basis for catalytic silencing.

S.S.Picaud, J.R.Muniz, A.Kramm, E.S.Pilka, G.Kochan, U.Oppermann, W.W.Yue.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 1.
Figure 1. Crystal structure of hCA VIII. (A) Ribbon diagram showing the secondary structure elements: -helices (red) and -strands (yellow). (B) C^ -superposition of hCA VIII (blue) and hCA XIII structures (cyan, PDB code 3DA2). The two loop regions that are unique in hCA VIII are shown in thick ribbons.

Figure 2.
Figure 2. Structural comparison of hCA VIII with the catalytic isozymes. (A) A close-up view showing the entrance of the hCA VIII cavity. hCA VIII is represented in blue ribbon, overlayed with a surface representation. (B) Active site of the CO[2]-bound hCA II (left, PDB code 3D92) and hCA VIII (right) structures. The hydrophobic CO[2] pocket is shown in pink surface. Spheres represent the Zn^2+ ion (black), water (red) and Cl^- ion (green). In the right panel, the CO[2] substrate from the hCA II structure (shown in dots) is superimposed onto the hCA VIII structure to illustrate the potential steric clash caused by I165 and I143 (green surface). (C) Schematic representation of the Zn^2+ coordination in hCA II (left), and Cl^- coordination in hCA VIII (right). Bond lengths are indicated in Å.

The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2009, 76, 507-511) copyright 2009.