PDBsum entry 2per

Electron transport

PDB id: 2per

Contents

Protein chain

222 a.a. *

Ligands

MNB

Waters ×68

* Residue conservation analysis

PDB id:

2per

Name:

Electron transport

Title:

Crystal structure of human chloride intracellular channel protein 2

Structure:

Chloride intracellular channel protein 2. Chain: a. Synonym: xap121. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Tissue: liver. Gene: clic2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.218 R-free: 0.258

Authors:

X.D.Su,Y.H.Liang,W.Mi

Key ref:

W.Mi et al. (2008). The crystal structure of human chloride intracellular channel protein 2: a disulfide bond with functional implications. Proteins, 71, 509-513. PubMed id: 18186468 DOI: 10.1002/prot.21922

Date:

03-Apr-07 Release date: 04-Mar-08

Protein chain

O15247  (CLIC2_HUMAN) -  Chloride intracellular channel protein 2 from Homo sapiens

Seq: 247 a.a.

Struc: 222 a.a.

Enzyme reactions

• Enzyme class 2: E.C.1.11.1.- - ?????
• Enzyme class 3: E.C.1.8.-.-

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1002/prot.21922

Proteins 71:509-513 (2008)

PubMed id: 18186468

The crystal structure of human chloride intracellular channel protein 2: a disulfide bond with functional implications.

W.Mi, Y.H.Liang, L.Li, X.D.Su.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 1.
Figure 1. Structure of human CLIC2. (A) Structure based sequence alignment of human CLIC1-6 and DHAR2, DHAR3 from A. thaliana. Identical and conserved residues are colored in red and yellow, respectively. CXXC motif is colored in cyan and marked by upwards triangles. The additional N-terminal sequences of CLIC5 and CLIC6 were truncated. (B) Overall structure of CLIC2. N-terminal domain is colored in red; C-terminal domain in green, the joint loop in yellow, the foot loop in blue, disulfide bond between Cys^30 and Cys^33 is showed as stick (yellow). (C) Structure superimposition of CLIC1 (yellow), CLIC4 (red) structures on CLIC2 (green). Disulfide bond in CLIC2 is showed in stick (yellow).

Figure 2.
Figure 2. Disulfide bond between Cys^30 and Cys^33 and the electrostatic potential surface of CLIC2. (A) Structure comparison between the N-terminal domain of CLIC2 (green) and pig glutaredoxin (magenta), poxviral glutaredoxin (cyan). The disulfide bond of CLIC2 is showed in stick (yellow). (B) 2Fo-Fc electron density map and stick diagram of the disulfide bond in human CLIC2. (C) Structure comparison of disulfide bonds between CLIC2 (green) with pig glutaredoxin (magenta), poxviral glutaredoxin (cyan). (D) The electrostatic potential surface of CLIC2 and foot loop insertion into the cavity of the neighbor molecule.

The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2008, 71, 509-513) copyright 2008.