PDBsum entry 2khc

RNA binding protein

PDB id: 2khc

Contents

Protein chain

118 a.a. *

* Residue conservation analysis

PDB id:

2khc

Name:

RNA binding protein

Title:

Bruno rrm3+

Structure:

Testis-specific rnp-type RNA binding protein. Chain: a. Fragment: residues 684-801. Engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: aret, bruno, cg31762. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

10 models

Authors:

A.M.Lyon,B.S.Reveal,P.M.Macdonald,D.W.Hoffman

Key ref:

A.M.Lyon et al. (2009). Bruno protein contains an expanded RNA recognition motif. Biochemistry, 48, 12202-12212. PubMed id: 19919093

Date:

01-Apr-09 Release date: 22-Dec-09

Protein chain

O18409  (O18409_DROME) -  Testis-specific RNP-type RNA binding protein from Drosophila melanogaster

Seq: 808 a.a.

Struc: 118 a.a.

Enzyme reactions

• Enzyme class: E.C.?

Biochemistry 48:12202-12212 (2009)

PubMed id: 19919093

Bruno protein contains an expanded RNA recognition motif.

A.M.Lyon, B.S.Reveal, P.M.Macdonald, D.W.Hoffman.

ABSTRACT

The RNA recognition motif (or RRM) is a ubiquitous RNA-binding module present in approximately 2% of the proteins encoded in the human genome. This work characterizes an expanded RRM, which is present in the Drosophila Bruno protein, and targets regulatory elements in the oskar mRNA through which Bruno controls translation. In this Bruno RRM, the deletion of 40 amino acids prior to the N-terminus of the canonical RRM resulted in a significantly decreased affinity of the protein for its RNA target. NMR spectroscopy showed that the expanded Bruno RRM contains the familiar RRM fold of four antiparallel beta-strands and two alpha-helices, preceded by a 10-residue loop that contacts helix alpha(1) and strand beta(2); additional amino acids at the N-terminus of the domain are relatively flexible in solution. NMR results also showed that a truncated form of the Bruno RRM, lacking the flexible N-terminal amino acids, forms a stable and complete canonical RRM, so that the loss of RNA binding activity cannot be attributed to disruption of the RRM fold. This expanded Bruno RRM provides a new example of the features that are important for RNA recognition by an RRM-containing protein.