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PDBsum entry 2khc
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RNA binding protein
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PDB id
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2khc
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References listed in PDB file
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Key reference
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Title
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Bruno protein contains an expanded RNA recognition motif.
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Authors
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A.M.Lyon,
B.S.Reveal,
P.M.Macdonald,
D.W.Hoffman.
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Ref.
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Biochemistry, 2009,
48,
12202-12212.
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PubMed id
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Abstract
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The RNA recognition motif (or RRM) is a ubiquitous RNA-binding module present in
approximately 2% of the proteins encoded in the human genome. This work
characterizes an expanded RRM, which is present in the Drosophila Bruno protein,
and targets regulatory elements in the oskar mRNA through which Bruno controls
translation. In this Bruno RRM, the deletion of 40 amino acids prior to the
N-terminus of the canonical RRM resulted in a significantly decreased affinity
of the protein for its RNA target. NMR spectroscopy showed that the expanded
Bruno RRM contains the familiar RRM fold of four antiparallel beta-strands and
two alpha-helices, preceded by a 10-residue loop that contacts helix alpha(1)
and strand beta(2); additional amino acids at the N-terminus of the domain are
relatively flexible in solution. NMR results also showed that a truncated form
of the Bruno RRM, lacking the flexible N-terminal amino acids, forms a stable
and complete canonical RRM, so that the loss of RNA binding activity cannot be
attributed to disruption of the RRM fold. This expanded Bruno RRM provides a new
example of the features that are important for RNA recognition by an
RRM-containing protein.
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