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* Residue conservation analysis
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PDB id:
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Cytokine
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Title:
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Structure of sdf1 in complex with the cxcr4 n-terminus containing no sulfotyrosines
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Structure:
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Stromal cell-derived factor 1. Chain: a, c. Fragment: sdf-1-alpha(3-67) domain. Synonym: sdf-1, c-x-c motif chemokine 12, pre-b cell growth- stimulating factor, pbsf, hirh. Engineered: yes. Mutation: yes. C-x-c chemokine receptor type 4. Chain: b, d.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: cxcl12, sdf1, sdf1a, sdf1b. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cxcr4.
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NMR struc:
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20 models
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Authors:
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B.F.Volkman,C.T.Veldkamp,F.C.Peterson
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Key ref:
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C.T.Veldkamp
et al.
(2008).
Structural basis of CXCR4 sulfotyrosine recognition by the chemokine SDF-1/CXCL12.
Sci Signal,
1,
ra4.
PubMed id:
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Date:
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24-Jan-08
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Release date:
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28-Oct-08
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PROCHECK
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Headers
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References
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Sci Signal
1:ra4
(2008)
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PubMed id:
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Structural basis of CXCR4 sulfotyrosine recognition by the chemokine SDF-1/CXCL12.
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C.T.Veldkamp,
C.Seibert,
F.C.Peterson,
N.B.De la Cruz,
J.C.Haugner,
H.Basnet,
T.P.Sakmar,
B.F.Volkman.
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ABSTRACT
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Stem cell homing and breast cancer metastasis are orchestrated by the chemokine
stromal cell-derived factor 1 (SDF-1) and its receptor CXCR4. Here, we report
the nuclear magnetic resonance structure of a constitutively dimeric SDF-1 in
complex with a CXCR4 fragment that contains three sulfotyrosine residues
important for a high-affinity ligand-receptor interaction. CXCR4 bridged the
SDF-1 dimer interface so that sulfotyrosines sTyr7 and sTyr12 of CXCR4 occupied
positively charged clefts on opposing chemokine subunits. Dimeric SDF-1 induced
intracellular Ca2+ mobilization but had no chemotactic activity; instead, it
prevented native SDF-1-induced chemotaxis, suggesting that it acted as a potent
partial agonist. Our work elucidates the structural basis for sulfotyrosine
recognition in the chemokine-receptor interaction and suggests a strategy for
CXCR4-targeted drug development.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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B.K.Kramp,
A.Sarabi,
R.R.Koenen,
and
C.Weber
(2011).
Heterophilic chemokine receptor interactions in chemokine signaling and biology.
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Exp Cell Res,
317,
655-663.
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B.Martín-Antonio,
M.Carmona,
J.Falantes,
E.Gil,
A.Baez,
M.Suarez,
P.Marín,
I.Espigado,
and
A.Urbano-Ispizua
(2011).
Impact of constitutional polymorphisms in VCAM1 and CD44 on CD34+ cell collection yield after administration of granulocyte colony-stimulating factor to healthy donors.
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Haematologica,
96,
102-109.
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C.L.Salanga,
and
T.M.Handel
(2011).
Chemokine oligomerization and interactions with receptors and glycosaminoglycans: the role of structural dynamics in function.
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Exp Cell Res,
317,
590-601.
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C.Zhong,
and
J.Ding
(2011).
New G-protein-coupled receptor structures provide insights into the recognition of CXCL12 and HIV-1 gp120 by CXCR4.
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Acta Biochim Biophys Sin (Shanghai),
43,
337-338.
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B.Wu,
E.Y.Chien,
C.D.Mol,
G.Fenalti,
W.Liu,
V.Katritch,
R.Abagyan,
A.Brooun,
P.Wells,
F.C.Bi,
D.J.Hamel,
P.Kuhn,
T.M.Handel,
V.Cherezov,
and
R.C.Stevens
(2010).
Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists.
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Science,
330,
1066-1071.
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PDB codes:
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D.H.Jones,
S.E.Cellitti,
X.Hao,
Q.Zhang,
M.Jahnz,
D.Summerer,
P.G.Schultz,
T.Uno,
and
B.H.Geierstanger
(2010).
Site-specific labeling of proteins with NMR-active unnatural amino acids.
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J Biomol NMR,
46,
89.
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J.L.Galzi,
M.Hachet-Haas,
D.Bonnet,
F.Daubeuf,
S.Lecat,
M.Hibert,
J.Haiech,
and
N.Frossard
(2010).
Neutralizing endogenous chemokines with small molecules. Principles and potential therapeutic applications.
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Pharmacol Ther,
126,
39-55.
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J.W.Murphy,
H.Yuan,
Y.Kong,
Y.Xiong,
and
E.J.Lolis
(2010).
Heterologous quaternary structure of CXCL12 and its relationship to the CC chemokine family.
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Proteins,
78,
1331-1337.
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PDB codes:
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O.C.Amadi,
M.L.Steinhauser,
Y.Nishi,
S.Chung,
R.D.Kamm,
A.P.McMahon,
and
R.T.Lee
(2010).
A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.
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Biomed Microdevices,
12,
1027-1041.
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S.T.Das,
L.Rajagopalan,
A.Guerrero-Plata,
J.Sai,
A.Richmond,
R.P.Garofalo,
and
K.Rajarathnam
(2010).
Monomeric and dimeric CXCL8 are both essential for in vivo neutrophil recruitment.
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PLoS One,
5,
e11754.
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A.Ravindran,
P.R.Joseph,
and
K.Rajarathnam
(2009).
Structural basis for differential binding of the interleukin-8 monomer and dimer to the CXCR1 N-domain: role of coupled interactions and dynamics.
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Biochemistry,
48,
8795-8805.
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C.C.Liu,
H.Choe,
M.Farzan,
V.V.Smider,
and
P.G.Schultz
(2009).
Mutagenesis and evolution of sulfated antibodies using an expanded genetic code.
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Biochemistry,
48,
8891-8898.
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C.C.Liu,
S.E.Cellitti,
B.H.Geierstanger,
and
P.G.Schultz
(2009).
Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code.
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Nat Protoc,
4,
1784-1789.
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C.H.Jen,
K.L.Moore,
and
J.A.Leary
(2009).
Pattern and temporal sequence of sulfation of CCR5 N-terminal peptides by tyrosylprotein sulfotransferase-2: an assessment of the effects of N-terminal residues.
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Biochemistry,
48,
5332-5338.
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C.T.Veldkamp,
J.J.Ziarek,
J.Su,
H.Basnet,
R.Lennertz,
J.J.Weiner,
F.C.Peterson,
J.E.Baker,
and
B.F.Volkman
(2009).
Monomeric structure of the cardioprotective chemokine SDF-1/CXCL12.
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Protein Sci,
18,
1359-1369.
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PDB codes:
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E.T.Brower,
A.Schön,
J.C.Klein,
and
E.Freire
(2009).
Binding thermodynamics of the N-terminal peptide of the CCR5 coreceptor to HIV-1 envelope glycoprotein gp120.
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Biochemistry,
48,
779-785.
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L.S.Simpson,
J.Z.Zhu,
T.S.Widlanski,
and
M.J.Stone
(2009).
Regulation of chemokine recognition by site-specific tyrosine sulfation of receptor peptides.
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Chem Biol,
16,
153-161.
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Y.Kofuku,
C.Yoshiura,
T.Ueda,
H.Terasawa,
T.Hirai,
S.Tominaga,
M.Hirose,
Y.Maeda,
H.Takahashi,
Y.Terashima,
K.Matsushima,
and
I.Shimada
(2009).
Structural basis of the interaction between chemokine stromal cell-derived factor-1/CXCL12 and its G-protein-coupled receptor CXCR4.
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J Biol Chem,
284,
35240-35250.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
