PDBsum entry 2ig2

Immunoglobulin

PDB id

2ig2

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Contents

			Protein chains

					216 a.a. *


					239 a.a. *

* Residue conservation analysis

PDB id:

2ig2

Links

Name:

Immunoglobulin

Title:

Dir primaerstruktur des kristallisierbaren monoklonalen immunoglobulins igg1 kol. Ii. Aminosaeuresequenz der l-kette, lambda- typ, subgruppe i (german)

Structure:

Igg1-lambda kol fab (light chain). Chain: l. Engineered: yes. Igg1-lambda kol fab (heavy chain). Chain: h. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606

Biol. unit:

Dimer (from PQS)

Resolution:

3.00Å

R-factor:

0.207

Authors:

M.Marquart,R.Huber

Key ref:

H.D.Kratzin et al. (1989). [The primary structure of crystallizable monoclonal immunoglobulin IgG1 Kol. II. Amino acid sequence of the L-chain, gamma-type, subgroup I]. Biol Chem Hoppe Seyler, 370, 263-272. PubMed id: 2713105

Date:

18-Apr-89

Release date:

12-Jul-89

Supersedes:

1ig2

PROCHECK

	Headers

	References

Protein chain

P0CG04 (IGLC1_HUMAN) - Immunoglobulin lambda constant 1 from Homo sapiens

Seq: Struc:					106 a.a. 216 a.a.

Protein chain

P01772 (HV333_HUMAN) - Immunoglobulin heavy variable 3-33 from Homo sapiens

Seq: Struc:					117 a.a. 239 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 19 residue positions (black crosses)

	Biol Chem Hoppe Seyler 370:263-272 (1989)
PubMed id: 2713105

[The primary structure of crystallizable monoclonal immunoglobulin IgG1 Kol. II. Amino acid sequence of the L-chain, gamma-type, subgroup I].
H.D.Kratzin, W.Palm, M.Stangel, W.E.Schmidt, J.Friedrich, N.Hilschmann.

ABSTRACT

The immunoglobulin Kol was the first intact antibody molecule which was characterized by high-resolution X-ray crystallography. Furthermore the complete amino-acid sequence of the heavy (H)-chain is known. Here we report the complete amino-acid sequence of the light (L)-chain of the monoclonal immunoglobulin Kol (IgG1). The polypeptide has an Mr of 22,781, consists of 216 amino acids and due to its structure is of the lambda-type. With the characteristic amino acids threonine, asparagine, threonine, glycine and lysine in positions 101, 114, 116, 154, and 165, respectively the Kol L-chain is of the Mcg isotype. With the proteins Mcg, Mot, Bur, Loc and Mem six myeloma-derived amino-acid sequences of the same isotype are known. The amino-acid sequence of the N-terminal variable part is characteristic of subgroup 1. This contribution completes the primary structure of IgG1 Kol.

Literature references that cite this PDB file's key reference

PubMed id

Reference

15320966

J.Sanders, J.Jeffreys, H.Depraetere, M.Evans, T.Richards, A.Kiddie, K.Brereton, L.D.Premawardhana, D.Y.Chirgadze, R.Núñez Miguel, T.L.Blundell, J.Furmaniak, and B.Rees Smith (2004).
Characteristics of a human monoclonal autoantibody to the thyrotropin receptor: sequence structure and function.

Thyroid, 14, 560-570.

12447901

P.A.Ramsland, and W.Farrugia (2002).
Crystal structures of human antibodies: a detailed and unfinished tapestry of immunoglobulin gene products.

J Mol Recognit, 15, 248-259.

8519975

M.Viswanathan, J.M.Anchin, P.R.Droupadi, C.Mandal, D.S.Linthicum, and S.Subramaniam (1995).
Structural predictions of the binding site architecture for monoclonal antibody NC6.8 using computer-aided molecular modeling, ligand binding, and spectroscopy.

Biophys J, 69, 741-753.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.