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PDBsum entry 2ig2
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Immunoglobulin
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PDB id
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2ig2
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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[The primary structure of crystallizable monoclonal immunoglobulin igg1 kol. Ii. Amino acid sequence of the l-Chain, Gamma-Type, Subgroup i].
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Authors
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H.D.Kratzin,
W.Palm,
M.Stangel,
W.E.Schmidt,
J.Friedrich,
N.Hilschmann.
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Ref.
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Biol Chem Hoppe Seyler, 1989,
370,
263-272.
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PubMed id
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Abstract
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The immunoglobulin Kol was the first intact antibody molecule which was
characterized by high-resolution X-ray crystallography. Furthermore the complete
amino-acid sequence of the heavy (H)-chain is known. Here we report the complete
amino-acid sequence of the light (L)-chain of the monoclonal immunoglobulin Kol
(IgG1). The polypeptide has an Mr of 22,781, consists of 216 amino acids and due
to its structure is of the lambda-type. With the characteristic amino acids
threonine, asparagine, threonine, glycine and lysine in positions 101, 114, 116,
154, and 165, respectively the Kol L-chain is of the Mcg isotype. With the
proteins Mcg, Mot, Bur, Loc and Mem six myeloma-derived amino-acid sequences of
the same isotype are known. The amino-acid sequence of the N-terminal variable
part is characteristic of subgroup 1. This contribution completes the primary
structure of IgG1 Kol.
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Secondary reference #1
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Title
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The three-Dimensional structure of antibodies
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Authors
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M.Marquart,
J.Deisenhofer.
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Ref.
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immunol today, 1982,
3,
160.
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Secondary reference #2
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Title
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Crystallographic refinement and atomic models of the intact immunoglobulin molecule kol and its antigen-Binding fragment at 3.0 a and 1.0 a resolution.
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Authors
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M.Marquart,
J.Deisenhofer,
R.Huber,
W.Palm.
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Ref.
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J Mol Biol, 1980,
141,
369-391.
[DOI no: ]
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PubMed id
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Figure 1.
FIG. 1. Final electron density of Kl Fab a model overlaid showing Phe451 to Pro454.
Po452 and Pro454 are in is conformation.
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Figure 9.
FIG. 9. Hypothetical IgG3 hinge segment.
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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Secondary reference #3
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Title
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Crystal structure of the human FAB fragment kol and its comparison with the intact kol molecule.
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Authors
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M.Matsushima,
M.Marquart,
T.A.Jones,
P.M.Colman,
K.Bartels,
R.Huber.
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Ref.
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J Mol Biol, 1978,
121,
441-459.
[DOI no: ]
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PubMed id
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Figure 3.
ARLE S
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Figure 5.
FIG. 5. C'' drawings of intact Kol Fab and ol IgG viewd long wn axis through the witrh
qhles.
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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Secondary reference #4
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Title
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Crystallographic structure studies of an igg molecule and an fc fragment.
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Authors
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R.Huber,
J.Deisenhofer,
P.M.Colman,
M.Matsushima,
W.Palm.
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Ref.
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Nature, 1976,
264,
415-420.
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PubMed id
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Secondary reference #5
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Title
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Structure of the human antibody molecule kol (immunoglobulin g1). An electron density map at 5 angstroms resolution
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Authors
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P.M.Colman,
J.Deisenhofer,
R.Huber,
W.Palm.
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Ref.
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j mol biol, 1976,
100,
257.
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