PDBsum entry 2fgo

Electron transport

PDB id

2fgo

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Contents

			Protein chain

					81 a.a. *

			Ligands

					SF4 ×2

			Waters ×141

* Residue conservation analysis

PDB id:

2fgo

Links

Name:

Electron transport

Title:

Structure of the 2[4fe-4s] ferredoxin from pseudomonas aeruginosa

Structure:

Ferredoxin. Chain: a. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.32Å

R-factor:

0.176

Authors:

P.Giastas,N.Pinotsis,I.M.Mavridis

Key ref:

P.Giastas et al. (2006). The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values. J Biol Inorg Chem, 11, 445-458. PubMed id: 16596388

Date:

22-Dec-05

Release date:

18-Apr-06

PROCHECK

	Headers

	References

Protein chain

Q9I6D2 (Q9I6D2_PSEAE) - Ferredoxin (4Fe-4S) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: Struc:					83 a.a. 81 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

	J Biol Inorg Chem 11:445-458 (2006)
PubMed id: 16596388

The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values.
P.Giastas, N.Pinotsis, G.Efthymiou, M.Wilmanns, P.Kyritsis, J.M.Moulis, I.M.Mavridis.

ABSTRACT

The structure of the 2[4Fe-4S] ferredoxin (PaFd) from Pseudomonas aeruginosa, which belongs to the Allochromatium vinosum (Alvin) subfamily, has been determined by X-ray crystallography at 1.32-A resolution, which is the highest up to now for a member of this subfamily of Fds. The main structural features of PaFd are similar to those of AlvinFd. However, the significantly higher resolution of the PaFd structure makes possible a reliable comparison with available high-resolution structures of [4Fe-4S]-containing Fds, in an effort to rationalize the unusual electrochemical properties of Alvin-like Fds. Three major factors contributing to the reduction potential values of [4Fe-4S]2+/+ clusters of Fds, namely, the surface accessibility of the clusters, the N-H...S hydrogen-bonding network, and the volume of the cavities hosting the clusters, are extensively discussed. The volume of the cavities is introduced in the present work for the first time, and can in part explain the very negative potential of cluster I of Alvin-like Fds.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20635418

B.S.Perrin, and T.Ichiye (2010).
Fold versus sequence effects on the driving force for protein-mediated electron transfer.

Proteins, 78, 2798-2808.

21029451

S.Elsen, G.Efthymiou, P.Peteinatos, G.Diallinas, P.Kyritsis, and J.M.Moulis (2010).
A bacteria-specific 2[4Fe-4S] ferredoxin is essential in Pseudomonas aeruginosa.

BMC Microbiol, 10, 271.

19290553

E.Saridakis, P.Giastas, G.Efthymiou, V.Thoma, J.M.Moulis, P.Kyritsis, and I.M.Mavridis (2009).
Insight into the protein and solvent contributions to the reduction potentials of [4Fe-4S]2+/+ clusters: crystal structures of the Allochromatium vinosum ferredoxin variants C57A and V13G and the homologous Escherichia coli ferredoxin.

J Biol Inorg Chem, 14, 783-799.

PDB codes:

2zvs 3eun 3exy

18160405

A.Karcher, A.Schele, and K.P.Hopfner (2008).
X-ray structure of the complete ABC enzyme ABCE1 from Pyrococcus abyssi.

J Biol Chem, 283, 7962-7971.

PDB code:

3bk7

17712580

H.Bönisch, C.L.Schmidt, P.Bianco, and R.Ladenstein (2007).
Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin: II. Introduction of an O-H...Sgamma-Fe hydrogen bond increased the reduction potential by 65 mV.

J Biol Inorg Chem, 12, 1163-1171.

PDB code:

2pya

17200753

K.Isele, F.Gigon, A.F.Williams, G.Bernardinelli, P.Franz, and S.Decurtins (2007).
Synthesis, structure and properties of {M4O4} cubanes containing nickel(II) and cobalt(II).

Dalton Trans, (), 332-341.

17611542

S.Dai, R.Friemann, D.A.Glauser, F.Bourquin, W.Manieri, P.Schürmann, and H.Eklund (2007).
Structural snapshots along the reaction pathway of ferredoxin-thioredoxin reductase.

Nature, 448, 92-96.

PDB codes:

2pu9 2puk 2puo 2pvd 2pvg 2pvo

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.