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PDBsum entry 2ewh
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* Residue conservation analysis
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Plos Biol
5:e144
(2007)
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PubMed id:
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Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome.
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Y.Tsai,
M.R.Sawaya,
G.C.Cannon,
F.Cai,
E.B.Williams,
S.Heinhorst,
C.A.Kerfeld,
T.O.Yeates.
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ABSTRACT
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The carboxysome is a bacterial organelle that functions to enhance the
efficiency of CO2 fixation by encapsulating the enzymes ribulose bisphosphate
carboxylase/oxygenase (RuBisCO) and carbonic anhydrase. The outer shell of the
carboxysome is reminiscent of a viral capsid, being constructed from many copies
of a few small proteins. Here we describe the structure of the shell protein
CsoS1A from the chemoautotrophic bacterium Halothiobacillus neapolitanus. The
CsoS1A protein forms hexameric units that pack tightly together to form a
molecular layer, which is perforated by narrow pores. Sulfate ions, soaked into
crystals of CsoS1A, are observed in the pores of the molecular layer, supporting
the idea that the pores could be the conduit for negatively charged metabolites
such as bicarbonate, which must cross the shell. The problem of diffusion across
a semiporous protein shell is discussed, with the conclusion that the shell is
sufficiently porous to allow adequate transport of small molecules. The
molecular layer formed by CsoS1A is similar to the recently observed layers
formed by cyanobacterial carboxysome shell proteins. This similarity supports
the argument that the layers observed represent the natural structure of the
facets of the carboxysome shell. Insights into carboxysome function are provided
by comparisons of the carboxysome shell to viral capsids, and a comparison of
its pores to the pores of transmembrane protein channels.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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T.O.Yeates,
M.C.Thompson,
and
T.A.Bobik
(2011).
The protein shells of bacterial microcompartment organelles.
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Curr Opin Struct Biol,
21,
223-231.
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C.A.Kerfeld,
S.Heinhorst,
and
G.C.Cannon
(2010).
Bacterial microcompartments.
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Annu Rev Microbiol,
64,
391-408.
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C.V.Iancu,
D.M.Morris,
Z.Dou,
S.Heinhorst,
G.C.Cannon,
and
G.J.Jensen
(2010).
Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells.
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J Mol Biol,
396,
105-117.
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S.Heinhorst,
and
G.C.Cannon
(2010).
Addressing microbial organelles: a short peptide directs enzymes to the interior.
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Proc Natl Acad Sci U S A,
107,
7627-7628.
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S.Tanaka,
M.R.Sawaya,
and
T.O.Yeates
(2010).
Structure and mechanisms of a protein-based organelle in Escherichia coli.
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Science,
327,
81-84.
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PDB codes:
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T.O.Yeates,
C.S.Crowley,
and
S.Tanaka
(2010).
Bacterial microcompartment organelles: protein shell structure and evolution.
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Annu Rev Biophys,
39,
185-205.
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F.Cai,
B.B.Menon,
G.C.Cannon,
K.J.Curry,
J.M.Shively,
and
S.Heinhorst
(2009).
The pentameric vertex proteins are necessary for the icosahedral carboxysome shell to function as a CO2 leakage barrier.
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PLoS One,
4,
e7521.
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K.A.Dryden,
C.S.Crowley,
S.Tanaka,
T.O.Yeates,
and
M.Yeager
(2009).
Two-dimensional crystals of carboxysome shell proteins recapitulate the hexagonal packing of three-dimensional crystals.
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Protein Sci,
18,
2629-2635.
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M.Beeby,
T.A.Bobik,
and
T.O.Yeates
(2009).
Exploiting genomic patterns to discover new supramolecular protein assemblies.
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Protein Sci,
18,
69-79.
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Y.Tsai,
M.R.Sawaya,
and
T.O.Yeates
(2009).
Analysis of lattice-translocation disorder in the layered hexagonal structure of carboxysome shell protein CsoS1C.
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Acta Crystallogr D Biol Crystallogr,
65,
980-988.
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PDB code:
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C.S.Crowley,
M.R.Sawaya,
T.A.Bobik,
and
T.O.Yeates
(2008).
Structure of the PduU shell protein from the Pdu microcompartment of Salmonella.
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Structure,
16,
1324-1332.
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PDB code:
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D.M.Morris,
and
G.J.Jensen
(2008).
Toward a biomechanical understanding of whole bacterial cells.
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Annu Rev Biochem,
77,
583-613.
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J.B.Parsons,
S.D.Dinesh,
E.Deery,
H.K.Leech,
A.A.Brindley,
D.Heldt,
S.Frank,
C.M.Smales,
H.Lünsdorf,
A.Rambach,
M.H.Gass,
A.Bleloch,
K.J.McClean,
A.W.Munro,
S.E.Rigby,
M.J.Warren,
and
M.B.Prentice
(2008).
Biochemical and structural insights into bacterial organelle form and biogenesis.
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J Biol Chem,
283,
14366-14375.
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M.Sutter,
D.Boehringer,
S.Gutmann,
S.Günther,
D.Prangishvili,
M.J.Loessner,
K.O.Stetter,
E.Weber-Ban,
and
N.Ban
(2008).
Structural basis of enzyme encapsulation into a bacterial nanocompartment.
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Nat Struct Mol Biol,
15,
939-947.
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PDB code:
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S.Cheng,
Y.Liu,
C.S.Crowley,
T.O.Yeates,
and
T.A.Bobik
(2008).
Bacterial microcompartments: their properties and paradoxes.
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Bioessays,
30,
1084-1095.
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S.Heinhorst,
and
G.C.Cannon
(2008).
A new, leaner and meaner bacterial organelle.
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Nat Struct Mol Biol,
15,
897-898.
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S.S.Cot,
A.K.So,
and
G.S.Espie
(2008).
A multiprotein bicarbonate dehydration complex essential to carboxysome function in cyanobacteria.
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J Bacteriol,
190,
936-945.
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S.Tanaka,
C.A.Kerfeld,
M.R.Sawaya,
F.Cai,
S.Heinhorst,
G.C.Cannon,
and
T.O.Yeates
(2008).
Atomic-level models of the bacterial carboxysome shell.
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Science,
319,
1083-1086.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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