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PDBsum entry 2doo
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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
2doo

 

 

 

 

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Contents
Protein chains
216 a.a. *
Ligands
C4H ×2
Metals
_ZN ×4
Waters ×279
* Residue conservation analysis
PDB id:
2doo
Name: Hydrolase
Title: The structure of imp-1 complexed with the detecting reagent (dansylc4sh) by a fluorescent probe
Structure: Beta-lactamase imp-1. Chain: a, b. Synonym: beta-lactamase, type ii, penicillinase, blaimp. Engineered: yes
Source: Serratia marcescens. Organism_taxid: 615. Gene: blaimp-1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.43Å     R-factor:   0.185     R-free:   0.225
Authors: H.Kurosaki,Y.Yamaguchi,H.Yasuzawa,W.Jin,Y.Yamagata,Y.Arakawa
Key ref: H.Kurosaki et al. (2006). Probing, inhibition, and crystallographic characterization of metallo-beta-lactamase (IMP-1) with fluorescent agents containing dansyl and thiol groups. Chemmedchem, 1, 969-972. PubMed id: 16937423
Date:
01-May-06     Release date:   28-Nov-06    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P52699  (BLAB_SERMA) -  Metallo-beta-lactamase type 2 from Serratia marcescens
Seq:
Struc: 		246 a.a.
216 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.5.2.6  - beta-lactamase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway: 		Penicillin Biosynthesis and Metabolism
      Reaction: a beta-lactam + H2O = a substituted beta-amino acid
      Cofactor: Zn(2+)

 

 
Chemmedchem 1:969-972 (2006)
PubMed id: 16937423  
 
 
Probing, inhibition, and crystallographic characterization of metallo-beta-lactamase (IMP-1) with fluorescent agents containing dansyl and thiol groups.
H.Kurosaki, Y.Yamaguchi, H.Yasuzawa, W.Jin, Y.Yamagata, Y.Arakawa.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20121112 P.Oelschlaeger, N.Ai, K.T.Duprez, W.J.Welsh, and J.H.Toney (2010).
Evolving carbapenemases: can medicinal chemists advance one step ahead of the coming storm?
  J Med Chem, 53, 3013-3027.  
18627129 D.Liu, J.Momb, P.W.Thomas, A.Moulin, G.A.Petsko, W.Fast, and D.Ringe (2008).
Mechanism of the quorum-quenching lactonase (AiiA) from Bacillus thuringiensis. 1. Product-bound structures.
  Biochemistry, 47, 7706-7714.
PDB codes: 3dha 3dhb 3dhc
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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