PDBsum entry 2cjj

DNA binding protein

PDB id

2cjj

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Contents

			Protein chain

					63 a.a. *

			Waters ×49

* Residue conservation analysis

PDB id:

2cjj

Links

Name:

DNA binding protein

Title:

Crystal structure of the myb domain of the rad transcription factor from antirrhinum majus

Structure:

Radialis. Chain: a. Engineered: yes. Other_details: 8 kda proteolytic fragment, myb domain

Source:

Antirrhinum majus. Garden snapdragon. Organism_taxid: 4151. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.90Å

R-factor:

0.216

R-free:

0.250

Authors:

C.E.M.Stevenson,N.Burton,M.M.Costa,U.Nath,R.A.Dixon,E.S.Coen, D.M.Lawson

Key ref:

C.E.Stevenson et al. (2006). Crystal structure of the MYB domain of the RAD transcription factor from Antirrhinum majus. Proteins, 65, 1041-1045. PubMed id: 17044043 DOI: 10.1002/prot.21136

Date:

04-Apr-06

Release date:

25-Oct-06

PROCHECK

	Headers

	References

Protein chain

Q58FS3 (RAD_ANTMA) - Transcription factor RADIALIS from Antirrhinum majus

Seq: Struc:					93 a.a. 63 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

DOI no: 10.1002/prot.21136	Proteins 65:1041-1045 (2006)
PubMed id: 17044043

Crystal structure of the MYB domain of the RAD transcription factor from Antirrhinum majus.
C.E.Stevenson, N.Burton, M.M.Costa, U.Nath, R.A.Dixon, E.S.Coen, D.M.Lawson.

ABSTRACT

No abstract given.

Selected figure(s)



	Figure 1. Figure 1. Stereo figure of the RAD fragment structure in ribbon representation colored with respect to sequence, starting from blue at the N-terminus through to red at the C-terminus. RAD is shown docked onto a double-stranded DNA fragment based on a superposition with the structure of the EH-DNA complex (PDB code 2HDD). The side chains of Trp 11, Trp 34, and Tyr 53 are also shown.		Figure 2. Figure 2. Structure-based sequence alignment of RAD against close structural homologues identified by DALI.[38] Only the sequences of the polypeptides present in the PDB entries are displayed. The positions of -helices are indicated by red shading and the locations of the W,W,W motifs are boxed. EH has a Phe in the third position according to the structural alignment, although the adjacent residue is a Trp (also boxed). Residues showing large chemical shifts in ARR10-B upon binding DNA are indicated with blue bars. Residues that contact DNA in the crystal structure of EH are also shown as bars: blue bars representing interactions with the phosphate backbone and green bars indicating interactions with the DNA bases.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21069464

F.Zhang, X.Liu, K.Zuo, X.Sun, and K.Tang (2011).
Molecular cloning and expression analysis of a novel SANT/MYB gene from Gossypium barbadense.

Mol Biol Rep, 38, 2329-2336.

19289599

D.G.Howarth, and M.J.Donoghue (2009).
Duplications and expression of DIVARICATA-like genes in dipsacales.

Mol Biol Evol, 26, 1245-1258.

19246540

Y.C.Lou, S.Y.Wei, M.Rajasekaran, C.C.Chou, H.M.Hsu, J.H.Tai, and C.Chen (2009).
NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis.

Nucleic Acids Res, 37, 2381-2394.

PDB codes:

2k9n 2kdz

17672842

C.E.Baxter, M.M.Costa, and E.S.Coen (2007).
Diversification and co-option of RAD-like genes in the evolution of floral asymmetry.

Plant J, 52, 105-113.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.