PDBsum entry 2b5u

Ribosome inhibitor, hydrolase

PDB id: 2b5u

Contents

Protein chains

470 a.a. *

84 a.a. *

Ligands

CIT ×2

Waters ×219

* Residue conservation analysis

PDB id:

2b5u

Name:

Ribosome inhibitor, hydrolase

Title:

Crystal structure of colicin e3 v206c mutant in complex with its immunity protein

Structure:

Colicin e3. Chain: a, c. Synonym: colicin e3 a chain, ribonuclease. Engineered: yes. Mutation: yes. Colicin e3 immunity protein. Chain: b, d. Synonym: imme3, microcin e3 immunity protein, colicin e3 chain b. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: ceac. Expressed in: escherichia coli str. K12 substr. W3110. Expression_system_taxid: 316407. Gene: imm, ceic, immb.

Biol. unit:

Tetramer (from PQS)

Resolution:

2.30Å R-factor: 0.259 R-free: 0.295

Authors:

A.Nallini Vijayarangan,S.Nithianantham,W.Nan,K.Jakes,M.Shoham

Key ref:

A.Nallini vijayarangan et al. Crystal structure of colicin e3 in complex with its immunity protein. To be published, .

Date:

29-Sep-05 Release date: 07-Nov-06

Protein chains

P00646  (CEA3_ECOLX) -  Colicin E3 from Escherichia coli

Seq: 551 a.a.

Struc: 470 a.a.*

Protein chains

P02984  (IMM3_ECOLX) -  Colicin E3 immunity protein from Escherichia coli

Seq: 85 a.a.

Struc: 84 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, C: E.C.4.6.1.- - ?????