PDBsum entry 2zq9

Hydrolase

PDB id: 2zq9

Contents

Protein chain

259 a.a. *

Ligands

SO4 ×2

CEO

CLS

SPA

Waters ×395

* Residue conservation analysis

PDB id:

2zq9

Name:

Hydrolase

Title:

Cephalothin acyl-intermediate structure of class a beta-lactamase toho-1 e166a/r274n/r276n triple mutant

Structure:

Beta-lactamase toho-1. Chain: a. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: bla. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.07Å R-factor: 0.133 R-free: 0.146

Authors:

Y.Nitanai,T.Shimamura,T.Uchiyama,Y.Ishii,M.Takehira,K.Yutani, H.Matsuzawa,M.Miyano

Key ref:

Y.Nitanai et al. Structural basis of extend spectrum beta-Lactamase in correlation of enzymatic kinetics and thermal stability of acyl-Intermediates. To be published, .

Date:

07-Aug-08 Release date: 28-Jul-09

Protein chain

Q47066  (BLT1_ECOLX) -  Beta-lactamase Toho-1 from Escherichia coli

Seq: 291 a.a.

Struc: 259 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)