PDBsum entry 2z2d

Hydrolase

PDB id: 2z2d

Contents

Protein chain

158 a.a. *

Ligands

HSI

Metals

_ZN ×2

_CA ×3

* Residue conservation analysis

PDB id:

2z2d

Name:

Hydrolase

Title:

Solution structure of human macrophage elastase (mmp-12) catalytic domain complexed with a gamma-keto butanoic acid inhibitor

Structure:

Macrophage metalloelastase. Chain: a. Fragment: catalytic domain, unp residues 106-263. Synonym: hme, matrix metalloproteinase-12, mmp-12, macrophage elastase, me. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mmp12. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

NMR struc:

15 models

Authors:

X.Zheng,L.Ou

Key ref:

X.Zheng and l.ou Solution structure of human macrophage elastase (mmp-12) catalytic domain complexed with a gamma-Keto butanoic acid inhibitor. To be published, .

Date:

18-May-07 Release date: 20-Nov-07

Protein chain

P39900  (MMP12_HUMAN) -  Macrophage metalloelastase from Homo sapiens

Seq: 470 a.a.

Struc: 158 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.24.65 - macrophage elastase.
• Reaction: Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin.
• Cofactor: Ca(2+); Zn(2+)