PDBsum entry 2yj4

Hydrolase

PDB id: 2yj4

Contents

Protein chains

248 a.a.

Ligands

AMP

PO4

ATP

Waters ×11

PDB id:

2yj4

Name:

Hydrolase

Title:

Conformational changes in the catalytic domain of the cpx-atpase copb- b upon nucleotide binding

Structure:

Copper-transporting atpase. Chain: a, b. Fragment: residues 383-645. Engineered: yes. Mutation: yes

Source:

Sulfolobus solfataricus. Organism_taxid: 273057. Strain: p2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.40Å R-factor: 0.223 R-free: 0.273

Authors:

C.Voellmecke,C.Schlicker,M.Luebben,E.Hofmann

Key ref:

C.Voellmecke et al. Conformational changes in the catalytic domain of the cpx-Atpase copb-B upon nucleotide binding. To be published, .

Date:

18-May-11 Release date: 30-May-12

Protein chains

Q97UU7  (Q97UU7_SULSO) -  Copper-transporting ATPase from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: 695 a.a.

Struc: 248 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.6.3.10 - Transferred entry: 7.2.2.19.
• Reaction: ATP + H₂O + H⁺(In) + K⁺(Out) = ADP + phosphate + H(+)(Out) + K(+)(In)

ATP + H(2)O + H(+)(In) + K(+)(Out) = ADP (Bound ligand (Het Group name = ATP) matches with 87.10% similarity) + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly) + H(+)(Out) + K(+)(In)

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site