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PDBsum entry 2uus
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Growth factor
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PDB id
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2uus
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Growth factor
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Title:
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Crystal structure of the rat fgf1-sucrose octasulfate (sos) complex.
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Structure:
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Heparin-binding growth factor 1. Chain: a, b. Fragment: residues 22-153. Synonym: hbgf-1, acidic fibroblast growth factor, afgf, rat fgf1, sucrose octasulfate. Engineered: yes
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Source:
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Rattus norvegicus. Rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.20Å
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R-factor:
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0.210
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R-free:
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0.277
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Authors:
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N.Kulahin,V.Kiselyov,A.Kochoyan,O.Kristensen,V.Berezin,E.Bock, M.Gajhede
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Key ref:
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N.Kulahin
et al.
(2008).
Dimerization effect of sucrose octasulfate on rat FGF1.
Acta Crystallogr Sect F Struct Biol Cryst Commun,
64,
448-452.
PubMed id:
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Date:
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07-Mar-07
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Release date:
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13-May-08
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PROCHECK
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Headers
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References
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P61149
(FGF1_RAT) -
Fibroblast growth factor 1 from Rattus norvegicus
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Seq:
Struc:
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155 a.a.
132 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Acta Crystallogr Sect F Struct Biol Cryst Commun
64:448-452
(2008)
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PubMed id:
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Dimerization effect of sucrose octasulfate on rat FGF1.
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N.Kulahin,
V.Kiselyov,
A.Kochoyan,
O.Kristensen,
J.S.Kastrup,
V.Berezin,
E.Bock,
M.Gajhede.
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ABSTRACT
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Fibroblast growth factors (FGFs) constitute a family of at least 23 structurally
related heparin-binding proteins that are involved in regulation of cell growth,
survival, differentiation and migration. Sucrose octasulfate (SOS), a chemical
analogue of heparin, has been demonstrated to activate FGF signalling pathways.
The structure of rat FGF1 crystallized in the presence of SOS has been
determined at 2.2 A resolution. SOS-mediated dimerization of FGF1 was observed,
which was further supported by gel-filtration experiments. The major
contributors to the sulfate-binding sites in rat FGF1 are Lys113, Lys118, Arg122
and Lys128. An arginine at position 116 is a consensus residue in mammalian FGF
molecules; however, it is a serine in rat FGF1. This difference may be important
for SOS-mediated FGF1 dimerization in rat.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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S.Sarilla,
S.Y.Habib,
D.V.Kravtsov,
A.Matafonov,
D.Gailani,
and
I.M.Verhamme
(2010).
Sucrose octasulfate selectively accelerates thrombin inactivation by heparin cofactor II.
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J Biol Chem,
285,
8278-8289.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
