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PDBsum entry 2uus
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Growth factor
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PDB id
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2uus
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References listed in PDB file
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Key reference
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Title
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Dimerization effect of sucrose octasulfate on rat fgf1.
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Authors
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N.Kulahin,
V.Kiselyov,
A.Kochoyan,
O.Kristensen,
J.S.Kastrup,
V.Berezin,
E.Bock,
M.Gajhede.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2008,
64,
448-452.
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PubMed id
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Abstract
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Fibroblast growth factors (FGFs) constitute a family of at least 23 structurally
related heparin-binding proteins that are involved in regulation of cell growth,
survival, differentiation and migration. Sucrose octasulfate (SOS), a chemical
analogue of heparin, has been demonstrated to activate FGF signalling pathways.
The structure of rat FGF1 crystallized in the presence of SOS has been
determined at 2.2 A resolution. SOS-mediated dimerization of FGF1 was observed,
which was further supported by gel-filtration experiments. The major
contributors to the sulfate-binding sites in rat FGF1 are Lys113, Lys118, Arg122
and Lys128. An arginine at position 116 is a consensus residue in mammalian FGF
molecules; however, it is a serine in rat FGF1. This difference may be important
for SOS-mediated FGF1 dimerization in rat.
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